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Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients

AuthorsFlechas, Ivonne D.; Cuéllar, Adriana; Cucunubá, Zulma M.; Rosas, Fernando; Velasco, Víctor; Steindel, Mario; Thomas, María del Carmen; López López, Manuel Carlos; González, John M.; Puerta, Concepción J.
Issue DateNov-2009
PublisherBioMed Central
CitationBMC Infectious Diseases 9(186): (2009)
AbstractBackground: Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude T. cruzi extract and three recombinant antigens: the T. cruzi kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the T. cruzi HSP-70 protein192-433, and the entire Trypanosoma rangeli HSP-70 protein. Methods: Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests. Results: The T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass.
Description11 pages, 6 figures.-- The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2334/9/186/pre pub
Publisher version (URL)http://dx.doi.org/10.1186/1471-2334-9-186
Appears in Collections:(IPBLN) Artículos
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