Preliminary observations on the use of isoelectric focusing of enzymes for the identification of Helicella species (Mollusca, Stylommatophora) from León Province (Spain)

Abstract

[EN]Samples representing H. bierzona, H. corderoi, H. itala, H. jamuzensis, H. madritensis, H. ordunensis y H. zaratei were collected from various parts of León Province. Five enzymes (malate dehydrogenase, glucose phosphate isomerase, phosphoglucomutase, acid phosphatase and hydroxybutyrate dehydrogenase) in homogenates of digestive gland and gonad of two hundred and twenty-eight snails were analysed by isolectric focusing in polyacrylamide gels. Good activity was obtained for all enzymes except hydroxybutyrate dehydrogenase. Consideration of the enzyme types identifiel for malate dehydrogenase and glucose phosphate isomerase allowed each species to be distinguished.

[ES]Se recolectaron muestras representativas de Helicella bierzona, H. corderoi, H. itala, H. jamuzensis, H. madritensis, H. ordunensis y H. zaratei, en varias partes de la provincia de León. Se analizaron cinco enzimas (malato deshidrogenasa, glucosa fosfato isomerasa, fosfoglucomutasa, ácido fosfatasa e hidroxibutirato deshidrogenasa) en homogeneizado de glándula digestiva y gónada de doscientos veintiocho caracoles, mediante la técnica de electroenfoque en gel de poliacrilamida. Se obtuvo buena actividad para todas las enzimas, excepto para hidroxibutirato deshidrogenasa. Si se consideran las enzimas tipo identificadas para malato deshidrogenasa y glucosa fosfato isomerasa, se pueden distinguir estas especies de Helicella, entre si.