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Different oxygen concentrations in lupin nodules functioning: genetic and physiological aspects

AuthorsGuasch, Luis; Fernández-Pascual, Mercedes ; Lucas, M. Mercedes ; Hernández-Jiménez, M. J.; Felipe, Mª Rosario de
Issue Date1997
PublisherKluwer Academic Publishers
CitationBiological Nitrogen Fixation for the 21st Century: 500-500 (1997)
AbstractThe effects of O2 concentrations (1, 21, 40% applied for 1, 3 and 10d) on nitrogenase activity (N2ase). O2 diffusion resistance (ODR), glycoprotein content, enod2 expression and nodule structure of Lupinus albus L plants have been studied. N2ase and ODR were measured using a flow-through system installed in a greenhouse. When 1% O2 was applied for 1d, N2ase was completely inhibited. However as O2 concentration was increased from 1% to 21%, nitrogenase activity was totally recovered. The recovery was only 30% after 3d of 1% O2 application and it was completely lost after 10d. When 40% O2 was applied during 1 and 3d a decrease in N2ase was detected, but after 10d N2ase was similar to the control. ODR increased highly with 3d of 40% O2 and it was double than control after 10d. Glycoprotein content decreased along 1% O2 treatment. In 40% O2 treated plants an increase was observed after 3d but after 10d there were no significant differences to the control. Expression of enod2 also diminished with 1% O2 and its evolution with 40% O2 followed a similar pattern to glycoprotein. A clear correlation between nodule functioning and structure was observed using different microscopies: At LM with 1% O2 nodule cortex changed progressively into an aerenchyma tissue. At 3d, middle cortex cells in the 40% O2 treatment presented an increase in size and their intercellular spaces (IS) were more occluded by glycoprotein (data confirmed by western-blot). Nodule structure was more similar to control after 10d of 40% O2. At TEM, 1% O2 bacteroids started to degrade after 3d, and after 10d bacteroids degradation was general. After 10d of 40% O2 bacteroids were larger (15%) and contained numerous polyhydroxybutirate grains. At Cryo-SEM, 1% O2 infected cells appeared highly vacuolated. The sizes of the IS of infected cells in 40% and 21%O2 were similar, although the volume of infected cells was bigger. So the IS volume/cell volume ratio diminished reducing O2 diffusion into the infected cells.
DescriptionTrabajo presentado en el 11th International Congress on Nitrogen Fixation, celebrado en Paris (Francia), del 20 al 25 de julio de 1997
Publisher version (URL)https://doi.org/10.1007/978-94-011-5159-7_310
Identifiersdoi: 10.1007/978-94-011-5159-7_310
isbn: 0-7923-4834-6
Appears in Collections:(ICA) Libros y partes de libros
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