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Open Access item Interplay Between Human Leukocyte Antigen Genes and the Microbial Colonization Process of the Newborn Intestine

Authors:Palma, Giada de
Capilla, Amalia
Nadal, Inmaculada
Nova, Esther
Pozo, Tamara
Varea, Vicente
Polanco, Isabel
Castillejo, Gemma
López, Antonio
Garrote, José Antonio
Calvo, Carmen
García Novo, M. D.
Cilleruelo, M. L.
Ribes-Koninckx, Carmen
Palau Martínez, Francesc
Sanz, Yolanda
Issue Date:Jan-2010
Publisher:Horizon Scientific Press
Citation:Current Issues in Molecular Biology 12: 1-10 (2010)
Abstract:Coeliac disease (CD) development involves genetic (HLA-DQ2/DQ8) and environmental factors. Herein, the influence of the HLA-DQ genotype on the gut colonization process of breast-fed children was determined. A cohort of 20 newborns, with at least one first-degree relative with CD, were classified according to their HLA-DQ genotype into high, intermediate and low genetic risk groups, showing 24-28%, 7-8% and less than 1% probability to develop CD, respectively. Faecal microbiota was analysed at 7 days, 1 and 4 months of children's age by fluorescence in situ hybridization. When considering all data, Gram-negative bacteria and Bacteroides-Prevotella group proportions were higher (P<0.05) in the high than in the intermediate and low genetic risk groups. E. coli, Streptococcus-Lactococcus, E. rectale-C. coccoides, sulphate-reducing bacteria, C. lituseburense and C. histolyticum group proportions were also significantly higher (P<0.05) in the high than in the low genetic risk group. Correlations between these bacterial groups and the genetic risk were also detected (P<0.05). In addition, the number and type of CD relative seemed to influence (P<0.050) these bacterial proportions in children at CD risk. At 4 months of age, similar relationships were established between the high genetic risk to develop CD and the proportions of Streptococcus-Lactococcus (P<0.05), E. rectale-C. coccoides (P<0.05), C. lituseburense (P<0.05), C. histolyticum (P<0.05), Bacteroides-Prevotella (P<0.10) groups and total Gram-negative bacteria (P<0.05). The results suggest a relationship between HLA-DQ genes and the gut microbial colonization process that could lead to a change in the way this disorder is investigated.
Description:11 pages, 3 tables, 2 figures.
Publisher version (URL):http://www.horizonpress.com/cimb/v/v12/1.pdf
URI:http://hdl.handle.net/10261/19926
ISSN:1467-3037
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