Brevundimonas canariensis sp. nov., isolated from roots of Triticum aestivum

Abstract

A bacterial strain designated GTAE24 was isolated from a root of wheat growing in soil from the Canary Islands, Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Brevundimonas with Brevundimonas abyssalis TAR-001 as its closest relative at 99.4% similarity. DNA–DNA hybridization studies showed an average of 38% relatedness between strain GTAE24 and the type strain of B. abyssalis. Cells were Gram-stain-negative and motile by polar flagella. The strain was positive for oxidase and weakly positive for catalase. Gelatin, starch and casein were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon source. Ubiquinone Q-10 was the predominant isoprenoid quinone and C03C97c/Cω6c (summed feature 8) and C were the major fatty acids. The major polar lipids were phosphatidylglycerol, 1,2-di-O-acyl-3-O-[D-glucopyranosyl-(1,4)-a-D-glucopyranuronosyl] glycerol, 1,2- diacyl-3-O-[6¢-phosphatidyl-a-D-glucopyranosyl] glycerol, 1,2-di-O-acyl-3-O-a-D-glucopyranosyl glycerol, and 1,2-di-O-acyl-3- O-a-D-glucopyranuronosyl glycerol. The DNA G+C content was 63.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain GTAE24 should be considered as representing a novel species of the genus Brevundimonas, for which the name Brevundimonas canariensis sp. nov. is proposed. The type strain is GTAE24 (=LMG 29500=CECT 9126).