Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/198640
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Demethoxy-Q, an intermediate of coenzyme Q biosynthesis, fails to support respiration in Saccharomyces cerevisiae and lacks antioxidant activity |
Autor: | Padilla, Sergio; Jonassen, Tanya; Jiménez-Hidalgo, María CSIC; Fernández-Ayala, Daniel J. M.; López-Lluch, Guillermo CSIC ORCID CVN ; Marbois, Beth; Navas, Plácido CSIC ORCID; Clarke, Catherine F.; Santos-Ocaña, Carlos CSIC ORCID | Fecha de publicación: | 2004 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 279(25): 25995-256004 (2004) | Resumen: | Caenorhabditis elegans clk-1 mutants cannot produce coenzyme Q(9) and instead accumulate demethoxy-Q(9) (DMQ(9)). DMQ(9) has been proposed to be responsible for the extended lifespan of clk-1 mutants, theoretically through its enhanced antioxidant properties and its decreased function in respiratory chain electron transport. In the present study, we assess the functional roles of DMQ(6) in the yeast Saccharomyces cerevisiae. Three mutations designed to mirror the clk-1 mutations of C. elegans were introduced into COQ7, the yeast homologue of clk-1: E233K, predicted to disrupt the di-iron carboxylate site considered essential for hydroxylase activity; L237Stop, a deletion of 36 amino acid residues from the carboxyl terminus; and P175Stop, a deletion of the carboxyl-terminal half of Coq7p. Growth on glycerol, quinone content, respiratory function, and response to oxidative stress were analyzed in each of the coq7 mutant strains. Yeast strains lacking Q(6) and producing solely DMQ were respiratory deficient and unable to support (6)either NADH-cytochrome c reductase or succinate-cytochrome c reductase activities. DMQ(6) failed to protect cells against oxidative stress generated by H(2)O(2) or linolenic acid. Thus, in the yeast model system, DMQ does not support respiratory activity and fails to act as an effective antioxidant. These results suggest that the life span extension observed in the C. elegans clk-1 mutants cannot be attributed to the presence of DMQ per se. | Versión del editor: | https://doi.org/10.1074/jbc.M400001200 | URI: | http://hdl.handle.net/10261/198640 | DOI: | 10.1074/jbc.M400001200 | ISSN: | 0021-9258 | E-ISSN: | 1083-351X |
Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
demethoactivi.pdf | 327,79 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
29
checked on 20-feb-2024
SCOPUSTM
Citations
60
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
57
checked on 24-feb-2024
Page view(s)
162
checked on 24-abr-2024
Download(s)
167
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.