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1,2-Dioleoylglycerol promotes calcium-induced fusion in phospholipid vesicles

AuthorsOrtiz, A.; Aranda, Francisco José; Villalaín, J.; San Martín, Carmen ; Micol, Vicente; Gómez-Fernandez, Juan C.
Phospholipid vesicles
Infrared spectroscopy
31p-nuclear magnetic resonance
Issue Date1992
CitationChemistry and Physics of Lipids 62: 215-224 (1992)
AbstractThe effect of 1,2-dioleoyglycerol (1,2-DOG) on the promotion of Ca2+-induced fusion of phosphatidylserine/phosphatidylcholine (PS/PC) vesicles was studied. 1,2-DOG is able to induce the mixing of membrane lipids at concentrations of 10 mol% without mixing of vesicular contents. At concentrations of 20 mol% or higher, 1,2-DOG promotes fusion, lipid and content mixing of LUV composed of an equimolar mixture of PS and PC, which otherwise are unable to fuse in the presence of Ca2+. Fusion was demonstrated by fluorescence assays monitoring mixing of aqueous vesicular contents and mixing of membrane lipids. Studies by Fourier transform infrared spectroscopy provided evidence for a fusion mechanism different to that of Ca2+-induced fusion of pure PS vesicles. Final equilibrium structures were characterized by 31P-NMR and freeze-fracture electron microscopy. Ca2+-induced fusion of 1,2-DOG containing vesicles is accompanied by the formation of isotropic structures which are shown to correspond to structures with lipidic particle morphology. The possible fusion mechanisms and implications are discussed. © 1992.
Identifiersissn: 0009-3084
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