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Título

Behavior of pectin during in vitro gastrointestinal digestion and effect on human colonic microbiota

AutorFerreira-Lazarte, Alvaro CSIC ORCID ; Gil-Sánchez, Irene CSIC; Cueva, Carolina CSIC ORCID ; Moreno, F. Javier CSIC ORCID ; Villamiel, Mar CSIC ORCID
Fecha de publicación2018
CitaciónXVIII Scientific Meeting of the Spanish Society of Chromatography and Related Techniques (2018)
ResumenRegulation of the microbiota through the consumption of prebiotics is a well-known concept nowadays. As it has been defined, prebiotics are a group of compounds with limited digestion that are selectively fermented by the gut microbiota conferring health benefits to the host. In this regard, pectin and its derivatives could be considered as prebiotic candidates. To evaluate this potential , influence on the microbiota has to be elucidated. One of the most relevant methodologies to determine the influence of potential prebiotics on the microbial composition is the quantitative polymerase chain reaction (qPCR), method which allows us to quantify the microbial population of bacteria through its DNA. In order to monitorise the behavior of pectin through the gastrointestinal tract, HPLC methods are considered the most useful technique due to its simplicity, selectivity and reliability. In this sense, the aim of this work was to evaluate the degradation of a commercial citrus pectin during the gastrointestinal digestion in a dynamic gastrointestinal simulator (simgi®), which simulates the stomach (ST), small intestine (SI) and ascendant, transverse and descendant colon (AC, TC, DC) by Size Exclusion Chromatography (HPLCSEC), and the influence on the microbial composition by qPCR. Citrus pectin samples were added to a nutritive medium (30 g/L) and were subjected to gastrointestinal digestion using the simgi®. Samples were withdrawn from all compartments during 5 weeks and, then, were centrifuged at 13,000g for 5 min; supernatant from feeding period was used for HPLC analysis of digested pectin whereas pellets were used for DNA extraction and bacterial quantification. TSK-GEL column G5000 PWXL. 7.8 x 300 mm, particle size 10 μm and G2500 PWXL, 7.8 x 300 mm, particle size 6, were consecutively used for the analysis and QIAamp DNA Stool Mini kit and ViiA7 PCR Systems were used for bacterial DNA extraction and detection, respectively. Pectin showed almost no changes in ST and SI when compared to the initial samples, whereas fermentation in the three colon compartments showed a high degradation with an increasing presence of low molecular weight carbohydrates (<18 kDa). Significant increase in Bifidobacterium, Bacteroides, Faecalibacterium prausnitzii and Enterobacteriaceae was observed after feeding with citrus pectin and an overall decrease was observed after the washout period. These results emphasize the potential benefits of pectin on gastrointestinal system even though further in vivo studies should be conducted.
DescripciónTrabajo presentado al XVIII Scientific Meeting of the Spanish Society of Chromatography and Related Techniques (SECyTA), celebrado en Granada del 2 al 4 de octubre de 2018.
URIhttp://hdl.handle.net/10261/195293
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