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Phenolic metabolites in human fecal samples: association with intestinal microbiota and serum biomarkers
|Authors:||González Solares, S.; Gutiérrez-Díaz, Isabel; Fernández-Navarro, Tania; Salazar, Nuria ; Bartolomé, Begoña ; Moreno-Arribas, M. Victoria ; González de los Reyes-Gavilán, Clara ; Gueimonde Fernández, Miguel|
|Citation:||9th Workshop SEPyP (2018)|
|Abstract:||[Introduction] Most of the health effects attributed to polyphenols may be associated with their phenolic microbial-derived metabolites, some of which could contribute to increase the antioxidant capacity of the plasma and/or to beneficially impact on gut microbial ecosystem and function. Therefore, the characterization of fecal microbial-derived phenolics is of interest for a better understanding of polyphenol metabolism by gut bacteria, and its consequences for human health.|
[Objectives] To examine, in a sample of mature adults without a declared pathology, the relationship between the excreted fecal phenolic metabolites, the composition and metabolic activity of the intestinal microbiota and selected blood parameters related to oxidative stress and inflammation.
[Methods] Blood and fecal samples from 71 healthy adults (70.83 ± 11.12 years old) were collected. In the fecal samples, PCR of the 16S rRNA gene was used for the quantification of major bacterial groups, analysis of SCFA was performed by gas chromatography, and UPLC-ESI-MS/MS was conducted for the analysis of fecal phenolic metabolites. In blood samples, the levels of immunological markers were quantified by flow cytometry except for TGF-β determined by ELISA.
[Results] Total phenolic metabolite content was inversely associated with the fecal levels of Bacteroides group, Clostridium cluster XIVa and propionate, and directly related to Lactobacillus group and acetate. The results obtained pointed to a direct association for TGF-β, IL-17 and IL-8 levels with the total phenolics excretion in feces, with independence of age, energy intake, physical activity or BMI. Phenylacetic and phenylpropionic acids were the major fecal phenolic compounds, accounting for 46.7 and 35.4%, respectively, of the total phenolics excreted.
[Conclusions] The reported association between the amounts of fecal phenolics metabolites, serum biomarkers and gut microbial composition will provide new approaches to promote the maintenance of health status.
|Description:||Resumen del trabajo presentado al IX Workshop on Probiotics, Prebiotics of the Spanish Society of Probiotics and Prebiotics (Sociedad Española de Probióticos y Prebióticos, SEPyP), celebrado en Zaragoza (España) del 15 al 16 de febrero de 2018.|
|Appears in Collections:||(IPLA) Comunicaciones congresos|
(CIAL) Comunicaciones congresos