English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/193455
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Comparative modulation of lncRNAs in wild-type and rag1- heterozygous mutant zebrafish exposed to immune challenge with spring viraemia of carp virus (SVCV)

AuthorsValenzuela-Muñoz, Valentina; Pereiro, Patricia ; Álvarez-Rodríguez, Margarita; Gallardo-Escárate, Cristian; Figueras Huerta, Antonio ; Novoa, Beatriz
Issue Date2019
PublisherNature Publishing Group
CitationScientific Reports 9: 14174 (2019)
AbstractAlthough the modulation of immune-related genes after viral infection has been widely described in vertebrates, the potential implications of non-coding RNAs (ncRNAs), especially long non-coding RNAs (lncRNAs), in immunity are still a nascent research field. The model species zebrafish could serve as a useful organism for studying the functionality of lncRNAs due to the numerous advantages of this teleost, including the existence of numerous mutant lines. In this work, we conducted a wholetranscriptome analysis of wild-type (WT) and heterozygous rag1 mutant (rag1+/−) zebrafish after infection with the pathogen spring viraemia of carp virus (SVCV). WT and rag1+/− zebrafish were infected with SVCV for 24 h. Kidney samples were sampled from infected and uninfected fish for transcriptome sequencing. From a total of 198,540 contigs, 12,165 putative lncRNAs were identified in zebrafish. Most of the putative lncRNAs were shared by the two zebrafish lines. However, by comparing the lncRNA profiles induced after SVCV infection in WT and rag1+/− fish, most of the lncRNAs that were significantly induced after viral challenge were exclusive to each line, reflecting a highly differential response to the virus. Analysis of the neighboring genes of lncRNAs that were exclusively modulated in WT revealed high representation of metabolism-related terms, whereas those from rag1+/− fish showed enrichment in terms related to the adaptive immune response, among others. On the other hand, genes involved in numerous antiviral processes surrounded commonly modulated lncRNAs, as expected. These results clearly indicate that after SVCV infection in zebrafish, the expression of an array of lncRNAs with functions in different aspects of immunity is induced
Description13 pages, 9 figures.-- This article is licensed under a Creative Commons Attribution 4.0 International License
Publisher version (URL)https://doi.org/10.1038/s41598-019-50766-0
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Comparative_modulation_2019.pdf5,1 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.