English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/19271
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Kaposi's sarcoma-associated herpesvirus protein LANA2 disrupts PML oncogenic domains and inhibits PML-mediated transcriptional repression of the survivin gene

AuthorsMarcos-Villar, Laura; Lopitz-Otsoa, Fernando; Gallego, Pedro; Muñoz-Fontela, César; González-Santamaría, José ; Campagna, Michela; Shou-Jiang, Gao; S. Rodríguez, Manuel; Rivas, Carmen
KeywordsKSHV
LANA2
PML
PODs
Sp100
Issue DateSep-2009
PublisherAmerican Society for Microbiology
CitationJournal of Virology 83(17): 8849-8858 (2009)
AbstractInfection by herpesviruses causes a dramatic disturbance of PML Oncogenic Domains (PODs) that has been suggested to be essential for viral lytic replication. Several proteins from Kaposi’s sarcoma-associated herpesvirus (KSHV) have been tested as putative PODs disrupting factors with negative results. Here, we show that LANA2, a viral protein that is absolutely required for the viability and proliferation of KSHV-infected primary effusion lymphoma (PEL) cells, increases the levels of SUMO2-ubiquitin-modified PML and induces the disruption of PODs by a proteasome-mediated mechanism. In addition, we demonstrate that this disruption is largely dependent both on the integrity of a SUMO-interaction motif in LANA2 and on the lysine 160 from PML. Moreover, silencing of the LANA2 expression in PEL cells by RNA interference led to an increase in the PML levels. Finally, we demonstrate that LANA2 relieves PML-mediated transcriptional repression of survivin, a protein that directly contributes to malignant progression of PEL. This represents the first example of inactivation of these important antiviral structures by KSHV.
Description10 pages, 7 figs, supplementary information may be found at http://jvi.asm.org/
URIhttp://hdl.handle.net/10261/19271
DOI10.1128/JVI.00339-09
Appears in Collections:(CNB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.