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dc.contributor.authorGarcía-Fernández, Pabloes_ES
dc.contributor.authorCastellanos-Martínez, Sheilaes_ES
dc.contributor.authorIglesias, Josées_ES
dc.contributor.authorOtero, J. J.es_ES
dc.contributor.authorGestal, C.es_ES
dc.date.accessioned2019-10-14T10:43:47Z-
dc.date.available2019-10-14T10:43:47Z-
dc.date.issued2016-
dc.identifier.citationJournal of Invertebrate Pathology 138: 57-62 (2016)es_ES
dc.identifier.issn0022-2011-
dc.identifier.urihttp://hdl.handle.net/10261/192579-
dc.description6 pages, 3 figures, 2 tableses_ES
dc.description.abstractThe common octopus, Octopus vulgaris is a new candidate species for aquaculture. However, rearing of octopus paralarvae is hampered by high mortality and poor growth rates that impede its entire culture. The study of genes involved in the octopus development and immune response capability could help to understand the key of paralarvae survival and thus, to complete the octopus life cycle. Quantitative real-time PCR (RT-qPCR) is the most frequently tool used to quantify the gene expression because of specificity and sensitivity. However, reliability of RT-qPCR requires the selection of appropriate normalization genes whose expression must be stable across the different experimental conditions of the study. Hence, the aim of the present work is to evaluate the stability of six candidate genes: β-actin (ACT), elongation factor 1-α (EF), ubiquitin (UBI), β-tubulin (TUB), glyceraldehyde 3-phosphate dehydrogenase (GADPH) and ribosomal RNA 18 (18S) in order to select the best reference gene. The stability of gene expression was analyzed using geNorm, NormFinder and Bestkeeper, in octopus paralarvae of seven developmental stages (embryo, paralarvae of 0, 10, 15, 20, 30 and 34 days) and paralarvae of 20 days after challenge with Vibrio lentus and Vibrio splendidus. The results were validated by measuring the expression of PGRP, a stimuli-specific gene. Our results showed UBI, EF and 18S as the most suitable reference genes during development of octopus paralarvae, and UBI, ACT and 18S for bacterial infection. These results provide a basis for further studies exploring molecular mechanism of their development and innate immune defensees_ES
dc.description.sponsorshipThis work was funded by the “Xunta de Galicia” – Spain 10PXIB402116PR research project, and “AGL20134910-C02-2R” from the Spanish Ministerio de Economia y Competitividad. S. Castellanos-Martínez thanks “CONACYT” – Mexico for her awarded scholarship, and P. García-Fernández thanks “Xunta de Galicia” for his predoctoral fellowshipes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/AGL2013-49101-C2-2-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectOctopus vulgarises_ES
dc.subjectParalarvaees_ES
dc.subjectReference genees_ES
dc.subjectRT-qPCRes_ES
dc.subjectDevelopmentes_ES
dc.subjectInfectiones_ES
dc.titleSelection of reliable reference genes for RT-qPCR studies in Octopus vulgaris paralarvae during development and immune-stimulationes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.jip.2016.06.003-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jip.2016.06.003es_ES
dc.identifier.e-issn1096-0805-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
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