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Scrippsiella acuminata versus Scrippsiella ramonii: A Physiological Comparison

AuthorsFagín, Elena; Bravo, Isabel; Garrido, J. L. ; Rodríguez, Francisco; Figueroa, Rosa Isabel
Cell cycle
Life cycle
Flow cytometry
Genome size
Issue Date2019
PublisherJohn Wiley & Sons
CitationCytometry - Part A 95(9): 1-12 (2019)
AbstractScrippsiella is a cosmopolitan dinoflagellate genus that is able to form Harmful Algal Blooms in coastal waters. The large physiological, morphological, and genetic variability that characterizes this genus suggest the existence of cryptic species. In this study, flow cytometric analyses were carried out to compare the cell cycle and life cycle of two Scrippsiella strains from two different species: Scrippsiella ramonii (VGO1053) and Scrippsiella acuminata (S3V). Both species were also investigated by internally transcribed spacer rDNA sequencing and high-performance liquid chromatography-based pigment analyses. The reddish-brown color of S. acuminata and yellowish-green hue of S. ramonii were consistent with the quantitative differences determined in their pigment profiles. Our results indicate that the cell cycle is light-controlled and that it differs in the two species. S-phase was detected during the light period in both, whereas the G2/M phase occurred during the light period in S. ramonii but under dark conditions in S. acuminata. The detection of 4C stages, mobile zygotes (planozygotes), and resting cysts in S. ramonii (nonclonal) provided convincing evidence of sexuality in this species. Sexual related processes were not found in the clonal S. acuminata strain, suggesting its heterothallic behavior (i.e., the need for outcrossing). The differences in the genome size of these species were examined as well
Description12 pages, 6 figures.-- Open access
Publisher version (URL)https://doi.org/10.1002/cyto.a.23849
Appears in Collections:(IIM) Artículos
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