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Título: | A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics |
Autor: | Berbel, Dàmaris; Cámara, Jordi; García, Ernesto CSIC ORCID ; Tubau, Fe; Guérin, François; Giard, Jean-Christophe; Domínguez, M. Ángeles; Cattoir, Vincent; Ardanuy, Carmen | Palabras clave: | GAS L phenotype LS(A)P phenotype Streptococcus pyogenes lnu(B) lsa(E) |
Fecha de publicación: | 30-ago-2019 | Editor: | Elsevier | Citación: | Int J Antimicrob Agents S0924-8579(19)30237-7 (2019) | Resumen: | A lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes [group A streptococcus (GAS)]. The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics. Antimicrobial susceptibility was tested using the microdilution broth method and the resistance phenotype was tested by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). Whole-genome sequencing revealed that the strain was ST10 and emm93, and had five resistance genes [lnu(B), ant(6)-Ia, aph(3')-III, tet(M) and dfrG]. The tet(M) gene was located in a Tn916-like transposon. The lsa(E)-lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Ia-sat4-aph(3')III] and lsa(E)-lnu(B) genes. This structure was not transferred by conjugation. This study identified a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS. | Descripción: | 19 p.-1 fig. | Versión del editor: | https://doi.org/10.1016/j.ijantimicag.2019.08.019 | URI: | http://hdl.handle.net/10261/192378 | DOI: | 10.1016/j.ijantimicag.2019.08.019 | ISSN: | 0924-8579 | E-ISSN: | 1872-7913 |
Aparece en las colecciones: | (CIB) Artículos |
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Berbel et al revised manuscript.pdf | Postprint | 5,69 MB | Adobe PDF | Visualizar/Abrir |
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