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Do metabolic and isotopic changes underpin physiological responses to drought in Medicago sativa plants?

AuthorsMolero Milán, Gemma; Roca, Regina; Mauve, Caroline; Cabrera-Bosquet, Llorenç; Tcherkez, Guillaume; Araus, José Luis; Nogués, Salvador; Aranjuelo, Iker
Issue Date18-Oct-2016
CitationXIII Simposio Hispano-Portugués de Relaciones Hídricas en las Plantas (2016)
AbstractDrought is one of the environmental factors most affecting crop productivity under Mediterranean conditions. The effect of drought on plant performance has been widely described at leaf and nodule level. However, less attention has been addressed to the plantnodule interaction and its implications in metabolites exchange during a regrowth period. For this purpose, physiological characterization and metabolite profiles of different plant organs and nodules were analyzed. In order to study in more detail how N metabolism was affected by water stress, the plants were labeled with N-enriched isotopic air (15N2) using especially designed chambers. Metabolite profile and isotopic composition analyses revealed that, drought induced major changes in the accumulation of amino acids (Pro, Asn, His, Lys and Cys), carbohydrates (sucrose, xylose and pinitol) and organic acids (fumarate, succinate and maleate) in the nodules in comparison with other organs. The lower 15N-labeling observed in Ser, compared with other amino acids, can be related with its high turnover rate, which in turn, indicates its potential implication in photorespiratory process. Isotopic analysis of amino acids also revealed that Pro synthesis in the nodule was a local response to drought and not associated with a feedback inhibition from the leaves. It is clear that isotopic approaches in combination with metabolic profiling are powerful tools to study the turnover rates of various metabolic intermediates and to predict metabolic origin of the compounds as well as to understand metabolic responses to drought.
DescriptionTrabajo presentado en el XIII Simposio Hispano-Portugués de Relaciones Hídricas en las Plantas: “Aprendiendo a optimizar el uso del agua en las plantas para hacer de nuestro entorno un ambiente más sostenible”, celebrado en Pamplona (España), del 18 al 20 de octubre de 2016.
Appears in Collections:(IDAB) Comunicaciones congresos
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