Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/19216
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Título : Sulfur assimilation by Oxyrrhis marina feeding on a 35S-DMSP-labelled prey
Autor : Salo, Violeta, Simó, Rafel, Vila-Costa, Maria, Calbet, Albert
Fecha de publicación : Dec-2009
Editor: Wiley-Blackwell
Resumen: A laboratory grazing experiment was conducted with the aim of quantifying the sulfur assimilation by a herbivore protist feeding on a dimethylsulfoniopropionate (DMSP)-containing phytoplankter. When supplied with dissolved 35S-DMSP, cultures of an axenic strain of the diatom Thalassiosira pseudonana took up 60–95% of the added radioisotope and accumulated it untransformed in the cytoplasm. Radiolabelled diatom cells were offered as prey to the heterotrophic dinoflagellate Oxyrrhis marina. After 32 h in the dark, all the prey had been grazed and digested, leaving only radiolabelled O. marina in the grazing bottles and thus providing an estimate of the percentage of DMSP-sulfur retained by the predator. Subsequent precipitation with cold trichloroacetic acid (TCA) provided the fraction of retained DMSP-S that had been assimilated into the micrograzer macromolecules. In parallel incubations with predator and dissolved 35S-DMSP only (no prey), O. marina (and their closely associated bacteria) took up the radiolabelled substrate osmotrophically to an activity of 0.04 dpm cell−1 and assimilated it all into macromolecules. By correcting grazing 35S-DMSP assimilation for osmotrophic 35S-DMSP assimilation, and comparing it with the ingested radioisotope, the percentage of ingested DMSP-sulfur retained and assimilated by the predator was determined to be 32 ± 4%. This is the first study that provides direct evidence that ingestion of a DMSP-containing prey supplies structural sulfur to a herbivore protist and that quantifies this assimilative supply at one-third of ingested DMSP
Descripción : 10 pages, 6 figures, 1 table
Versión del editor: http://dx.doi.org/10.1111/j.1462-2920.2009.02011.x
URI : http://hdl.handle.net/10261/19216
ISSN: 1462-2912
DOI: 10.1111/j.1462-2920.2009.02011.x
Citación : Environmental Microbiology 11(12): 3063-3072 (2009)
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