English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/191750
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Rapid and specific detection of Salmonella infections using chemically modified nucleic acid probes

AuthorsMachado, Isabel; Garrido, Victoria ; Hernandez, Luiza I.; Botero, Juliana; Bastida, Nora; San Román, Beatriz ; Grilló, María Jesús ; Hernandez, Frank J.
KeywordsSalmonella
Nucleic acid probes
Detection system
Nucleases
Issue Date25-Apr-2019
PublisherElsevier
CitationAnalytica Chimica Acta 1054: 157-166 (2019)
AbstractSalmonella is a leading source of bacterial foodborne illness in humans, causing gastroenteritis outbreaks with bacteraemia occurrences that can lead to clinical complications and death. Eggs, poultry and pig products are considered as the main carriers of the pathogenic Salmonella for humans. To prevent this relevant zoonosis, key changes in food safety regulations were undertaken to improve controls in the food production chain. Despite these measures, large outbreaks of salmonellosis were reported worldwide in the last decade. Thus, new strategies for Salmonella detection are a priority for both, food safety and public health authorities. Such detection systems should provide significant reduction in diagnostic time (hours) compared to the currently available methods (days). Herein, we report on the discovery and characterization of nucleic acid probes for the sensitive and specific detection of live Salmonella within less than 8 h of incubation. We are the first to postulate the nuclease activity derived from Salmonella as biomarker of infection and its utility to develop innovative detection strategies. Our results have shown the screening and identification of two oligonucleotide sequences (substrates) as the most promising probes for detecting Salmonella – Sal-3 and Sal-5. The detection limits for both probes were determined with the reference Salmonella Typhimurium (STM 1) and Salmonella Enteritidis (SE 1) cultures. Sal-3 has reported LOD values around 105 CFU mL−1 for STM 1 and 104 CFU mL−1 for SE 1, while Sal-5 proves to be a slightly better probe, with LODs of 104 CFU mL−1 for STM 1 and 104 CFU mL−1 for SE 1. Both selected probes have shown the capability to recognize 49 out of 51 different Salmonella serotypes tested in vitro and the most frequent serotypes in porcine mesenteric lymph nodes as a standard sample used in fattening-pig salmonellosis baseline studies. Notably, our results showed 100% correlation between nuclease detection and the PCR-InvA or ISO-6579 standard method, underlining the great potential of this innovative nucleic acids technology to be implemented as a rapid method for food safety testing.
Publisher version (URL)https://doi.org/10.1016/j.aca.2018.12.027
URIhttp://hdl.handle.net/10261/191750
DOI10.1016/j.aca.2018.12.027
ISSN0003-2670
Appears in Collections:(IDAB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.