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dc.contributor.authorPicard-Sánchez, Amparoes_ES
dc.contributor.authorPiazzon de Haro, María Carlaes_ES
dc.contributor.authorEstensoro, Itziares_ES
dc.contributor.authorPozo, R. deles_ES
dc.contributor.authorPalenzuela, Oswaldoes_ES
dc.contributor.authorSitjà-Bobadilla, Ariadnaes_ES
dc.identifier.citation8th ISAAH (2018)es_ES
dc.descriptionTrabajo presentado en el 8th International Symposium on Aquatic Animal Health (ISAAH), celebrado en Charlottetown (Canadá), del 2 al 8 de septiembre de 2018es_ES
dc.description.abstractThe intestinal myxosporean parasite Enteromyxum leei causes chronic catarrhal enteritis in gilthead sea bream (GSB, Sparus aurata) leading to intestinal dysfunction, poor growth, and higher susceptibility to handling and stress. It entails severe economic losses to the aquaculture sector. Previous observations in our lab showed that fish that recovered from an E. leei infection did not get infected upon re-exposure, hinting towards the possibility of protective immunization of GSB against E. leei. To study this in more detail, we re-challenged putative resistant (R) GSB that recovered from this myxozoan infection, by exposure to E. leei effluent infected water. Another group of naïve (N) GSB (never exposed to the parasite) was also challenged. Both fish groups were sampled at 0, 61 and 86 days post-exposure (p.e.) and different specific and non-specific humoral factors were measured in serum, such as the total peroxidase activity, total IgM and IgT (by ELISA), and the presence and quantity of specific antibodies against the parasite by immunohistochemistry (IHC). The expression of a panel of immune-related genes was analysed in head kidney, anterior and posterior intestines of N and R fish after 86 days p.e. At this final sampling point, 83.3% of N fish vs 0% of R fish tested positive for E. leei by histology. The total peroxidase activity decreased with the progression of the infection in both groups, but only significantly in R fish, probably due to a higher consumption of this enzyme to fight the parasite. Total IgT and IgM levels did not significantly differ between groups. However, IHC evidenced that R fish had a higher initial level of specific anti-E. leei IgM than N fish. The PCR array showed a differential response between N and R fish and among tissues. R fish had significant up-regulation of IgM, IgT, il10 and gzmA and down-regulation of il1ß in anterior intestine. Complement (c3 and fcl) and the anti-protease alfa2m were significantly higher expressed in R posterior intestine and head kidney. However, the anti-protease lcpI was down-regulated in all tissues of R fish when compared to N. The higher initial pool of specific circulating antibodies against the parasite, together with the higher expression of Igs in anterior intestine, the higher cytotoxic activity in the whole target organ and the systemic increase in complement (lectin pathway) seem to be crucial to resist E. leei reinfection. Hence, this acquired protective immunity sets the grounds for the development of a vaccine or the production of recombinant antibodies against E. leei. This study was supported by EU H2020 program and by the Spanish Ministry of Economy and Competitiveness through ParaFishControl (634429) and AGL-2013-48560-R research projects, respectively. This publication reflects the views only of the authors, and the European Commission cannot be held responsible for any use which may be made of the information contained therein. MCP was contracted under CSIC PIE project no. 201740E013 and IE was recipient of APOSTD/2016/037 grant by the Generalitat Valenciana.es_ES
dc.description.sponsorshipThis study was supported by EU H2020 program and by the Spanish Ministry of Economy and Competitiveness through ParaFishControl (634429) and AGL-2013-48560-R research projects, respectively.-
dc.titleImmune players in acquired protection to enteromyxum leei (Myxozoa) in gilthead sea bream, Sparus aurata (Teleostei: Perciformes)es_ES
dc.typecomunicación de congresoes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidPiazzon de Haro, María Carla [0000-0002-4949-8984]es_ES
Appears in Collections:(IATS) Comunicaciones congresos
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