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Dietary amino acids inclusion in fishmeal-free diet for gilthead seabream (Sparus aurata) juveniles induces opposite effects depending on feeding time

AuthorsRamos-Pinto, L.; Martos-Sitcha, Juan Antonio ; Engrola, Sofía; Pérez-Sánchez, Jaume ; Silva, T. S.; Costas, Benjamín; Reis, D. B.; Calduch-Giner, Josep A. ; Conceição, Luis E. C.; Dias, Jorge
Issue Date3-Jun-2018
Citation18th ISFNF (2018)
AbstractThe concept of maintaining animal health through the best possible nutrition is well-accepted in modern animal farming, and amino acids appear to be good candidates as functional additives to improve health. The present study aimed to explore short and long-term effects of dietary amino acids (AA) supplementation on the gilthead seabream growth performance and immune status, in the context of a challenging fishmeal-free formulation. Triplicate groups of fish (13.3±0.3g) with a fishmeal diet background were either fed a control diet with an extreme formulation (0% fishmeal) but meeting the AA requirements (CTRL), or the CTRL diet with a 2% or a 9% surplus of histidine, threonine and tryptophan (HTW2 and HTW9, respectively). After 2 and 12 weeks of feeding, fish samples of head-kidney, liver and muscle were collected for gene expression, whereas plasma was suited for humoral immune parameters. There were no differences in final body weight among treatments. However, there was a tendency for an impaired growth performance in fish fed HTW9 after 12 weeks, which may be linked to an increased FCR at early stages in this group. The expression of a panel of 29-32 genes specific for muscle, liver or head-kidney confirms an effect due to the treatments across time. A two-way ANOVA analysis revealed that 15-24 genes vary significantly depending on the tissue, and the multivariate analysis of all shows that around 83% of variation is explained by the two first principal components (PC). In this regard, PC1 (60.3%) justified the effect of individual variability within diet mostly due to HTW9 at early stages, whereas PC2 (22.8%) represents a clear time effect. Particularly in the case of HK, fish fed HTW9 displayed an immunostimulated state at 2 weeks. No major differences were observed in plasma humoral parameters, with only an increase in antiprotease and peroxidase activities after 12 weeks regardless of dietary treatment. Overall, dietary AA supplementation might improve the seabream immune status over a short-term period. Hence, the use of nutraceutical feeds is especially relevant before/during/after exposure to stress, taking into account that these putative advantageous effects seem to disappear after a long-term feeding period.
DescriptionTrabajo presentado en el 18th International Symposium on Fish Nutrition and Feeding (ISFNF), celebrado en Las Palmas de Gran Canaria (España), del 3 al 07 de junio de 2018
Appears in Collections:(IATS) Comunicaciones congresos
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