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Effect of cationic amino (PS-NH2) polystyrene nanoparticles in brine shrimp Artemia franciscana nauplii: biochemical and molecular responses

AuthorsVaró, Inmaculada ; Perini, Deborah Aurora; Bergami, Elisa; Vannuccini, Maria L.; Corsi, Ilaria
KeywordsPolystyrene nanoparticles
Artemia franciscana
Toxicity, biomarkers
Issue Date13-May-2018
CitationSETAC Europe 28th Annual Meeting (2018)
AbstractThe accumulation of plastic litter on beaches and open oceans has been identified as one of the major threats in marine ecosystems worldwide. Laboratory experiments have proved that the formation of nano-sized plastics during the polymer degradation may reach marine ecosystem, considered as the most in danger. In present study, the effect of 50 nm cationic amino polystyrene (PS-NH2) was investigated in nauplii of Artemia franciscana, which is commonly used as aquatic model organism in toxicity tests. Acute toxicity tests were performed on nauplii exposed to sub-lethal suspensions of PS-NH2 (0.1, 1 and 10 ¿g/mL) in natural sea water (NSW) for 48 hours. The toxicity was evaluated by measuring growth and several biomarkers as carboxylesterase (CbE), glutathione-S-transferase (GST), cholinesterase (ChE), heat shock protein (HSP70), lipid peroxidation (LP) and catalase (CAT), involved in important physiological processes, such as biotransformation of xenobiotics, neuronal transmission and oxidative stress. The effect of PS-NH2 (at 0.1 and 1 ¿g/mL) on the expression of genes related to metabolism, biosynthesis and embryogenesis during the development of brine shrimp was also investigated. Genes included HSP26, HSP70, mitochondrial uncoupling protein 2 (UCP2), chaperonin-containing TCP (TCP) and late embryogenesis abundant (LEA). Acute exposure to sub-lethal suspensions PS-NH2 caused a significant decrease in growth in A. franciscana nauplii, as well as significant changes in all biomarkers studied, except for LP. A significant up-regulation of HSP26 and HSP70 was observed in nauplii exposed to 1 ¿g/mL of PS-NH2 as well as the modulation of TCP, the latter not significant. This supports the results obtained from biomarkers, suggesting a stress response and potential apoptotic pathway following PS-NH2 exposure. On the contrary, no significant effect on gene expression related to the brine shrimp¿s metabolism (UCP2) was observed, and LEA was significantly modulated only at the lowest concentration tested. These findings indicate that stress-related responses are taking place in exposed nauplii after acute exposure to sub-lethal suspensions of PS-NH2, and confirm the general concern about PS-NH2 and their ability to represent an ecological treat for marine organisms. Given the increasing levels of plastic pollution in the oceans, additional studies should be done considering long-term exposure to analyze the potential risk of nano-sized plastics in marine environments.
DescriptionTrabajo presentado en el Society of Environmental Toxicology and Chemistry (SETAC) Europe 28th Annual Meeting, celebrado en Roma (Italia), del 13 al 17 de mayo de 2018
Appears in Collections:(IATS) Comunicaciones congresos
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