English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/191371
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

The use of stable and unstable green fluorescent proteins for studies in two bacterial models: Agrobacterium tumefaciens and Xanthomonas campestris pv. campestris

AuthorsSabuquillo, Pilar; Gea, Adela; Matas, Isabel M. ; Ramos, Cayo; Cubero, Jaime
KeywordsGFP
Black rot
Crown gall
Plant disease
Plant pathology
Bacterial fitness
Issue DateMay-2017
PublisherSpringer
CitationArchives of Microbiology 119(4): 581–590 (2017)
AbstractFluorescent proteins have been used to track plant pathogens to understand their host interactions. To be useful, the transgenic pathogens must present similar behaviour than the wild-type isolates. Herein, a GFP marker was used to transform two plant pathogenic bacteria, Agrobacterium and Xanthomonas, to localize and track the bacteria during infection. The transgenic bacteria were evaluated to determine whether they showed the same fitness than the wild-type strains or whether the expression of the GFP protein interfered in the bacterial activity. In Agrobacterium, the plasmid used for transformation was stable in the bacteria and the strain kept the virulence, while Xanthomonas was not able to conserve the plasmid and transformed strains showed virulence variations compared to wild-type strains. Although marking bacteria with GFP to track infection in plants is a common issue, works to validate the transgenic strains and corroborate their fitness are not usual. Results, presented here, confirm the importance of proper fitness tests on the marked strains before performing localization assays, to avoid underestimation of the microbe population or possible artificial effects in its interaction with the plant.
Publisher version (URL)https://doi.org/10.1007/s00203-016-1327-0
URIhttp://hdl.handle.net/10261/191371
DOI10.1007/s00203-016-1327-0
ISSN0302-8933
E-ISSN1432-072X
Appears in Collections:(IHSM) Artículos
(IDAB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.