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Título : Studies on the expression of recombinant fuculose-1-phosphate aldolase in E. coli
Autor : Durany, Olga, Caminal, Glòria, Mas, Carles de, López-Santín, Josep
Palabras clave : Recombinant aldolases
Growth medium
IPTG induction
E. coli
Fecha de publicación : 30-Jul-2004
Editor: Elsevier
Citación : Process Biochemistry 39(11): 1677-1684 (2004)
Resumen: Fuculose-1-phosphate aldolase (Fuc-1-PA) is a dihydroxyacetone phosphate (DHAP) dependent aldolase with potential application in chiral synthesis. The influence of the growth medium on the expression of the enzyme in Escherichia coli has been studied. Complex LB medium, a defined medium (MD) and a semi-complex medium (MSC) have been compared in order to maximize aldolase production. The defined medium produced highest expression levels (700 activity units (AU)/g of dry cell weight (DCW)). The optimal induced isopropyl-β-thiogalactopyranoside (IPTG) concentration of 100 μM produces in the MD medium of 41 μmol/g dry cell weight of enzyme.
Descripción : 8 pages, 5 figures, 3 tables.-- Available online Sep 23, 2003.
Versión del editor:
ISSN: 1359-5113
DOI: 10.1016/S0032-9592(03)00302-9
Citación : Process Biochemistry 39(11): 1677-1684 (2004)
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