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Closed Access item Studies on the expression of recombinant fuculose-1-phosphate aldolase in E. coli

Authors:Durany, Olga
Caminal, Glòria
Mas, Carles de
López-Santín, Josep
Keywords:Recombinant aldolases, Fuc-1-PA, Growth medium, IPTG induction, E. coli
Issue Date:30-Jul-2004
Citation:Process Biochemistry 39(11): 1677-1684 (2004)
Abstract:Fuculose-1-phosphate aldolase (Fuc-1-PA) is a dihydroxyacetone phosphate (DHAP) dependent aldolase with potential application in chiral synthesis. The influence of the growth medium on the expression of the enzyme in Escherichia coli has been studied. Complex LB medium, a defined medium (MD) and a semi-complex medium (MSC) have been compared in order to maximize aldolase production. The defined medium produced highest expression levels (700 activity units (AU)/g of dry cell weight (DCW)). The optimal induced isopropyl-β-thiogalactopyranoside (IPTG) concentration of 100 μM produces in the MD medium of 41 μmol/g dry cell weight of enzyme.
Description:8 pages, 5 figures, 3 tables.-- Available online Sep 23, 2003.
Publisher version (URL):http://dx.doi.org/10.1016/S0032-9592(03)00302-9
Appears in Collections:(IQAC) Artículos

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