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Avaliação do estado fisiológico das paralarvas de Octopus vulgaris resultantes de ovos incubados a diferentes temperaturas, através de biomarcadores clássicos

AuthorsMagro, Martim; Navarro, Juan Carlos ; Hontoria, Francisco ; Garrido, Diego; Almansa, E.; Varó, Inmaculada
Issue Date13-Oct-2019
CitationXV Congreso Nacional de Acuicultura (2015)
I Congreso Ibérico de Acuicultura (2015)
AbstractOctopus vulgaris rearing/production presents several problems, mainly during the first stages, from eggs incubation to paralarvae rearing. In the last years, several research groups have been doing an effort to produce O. vulgaris in aquaculture focused on larval rearing phase, in order to solve these bottlenecks. In this sense, the present study aims to analyse the effect of different temperatures on common octopus embryonic development from one single female brood; as well as, larval viability and biomarkers of growth, physiological stress, antioxidant defences and neuronal activity (RNA/DNA, HSP70, GST and AChE, respectively). O. vulgaris eggs were incubated from stage XV to hatchlings at 19ºC and 22ºC, and then were maintained at room temperature (22±1ºC) for 14 days. Survival, specific growth rate, biomass and dry weight were measured over time. Samples from 0 and 14 days were taken in order to analyse biomarkers individually. Only HSP70 was analysed using a pool of 7-8 paralarvae. The results showed differences between temperatures in terms of survival and specific growth rate. RNA/DNA ratios and GST (0 and 14 days), and HSP70 (0, 7 and 14 days) showed differences between ages at different temperatures. No significant differences were found in AChE activity between groups (age and temperatures). The results point out at the RNA/DNA ratio, GST and HSP70 as sensitive biomarkers for growth, thermal stress and antioxidant defences in paralarvae. However, growth and temperature did not alter the neurotransmission system of the paralarvae.
DescriptionTrabajo presentado en el XV Congreso Nacional de Acuicultura y el I Congreso Ibérico de Acuicultura, celebrados en Huelva (España) del 13 al 16 de octubre de 2015
Appears in Collections:(IATS) Comunicaciones congresos
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