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Título: | Importance of the Support Properties for Immobilization or Purification of Enzymes |
Autor: | Sousa dos Santos, José Cleiton; Barbosa, Oveimar CSIC ORCID; Ortiz, Claudia; Berenguer Murcia, A.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCID | Palabras clave: | Superficial density of reactive groups Stabilization One‐point attachment Immobilization Enzyme purification Multipoint attachment |
Fecha de publicación: | 17-ago-2015 | Editor: | Wiley-VCH | Citación: | ChemCatChem 7(16): 2413-2432 (2015) | Resumen: | Immobilization and purification of enzymes are usual requirements for their industrial use. Both purification and immobilization have a common factor: they use a solid activated support. Using a support for enzyme purification means having mild conditions for enzyme release and a selective enzyme–support interaction is interesting. When using a support for immobilization, however, enzyme desorption is a problem. The improvement of enzyme features through immobilization is a usual objective (e.g., stability, selectivity). Thus, a support designed for enzyme purification and a support designed for enzyme immobilization may differ significantly. In this review, we will focus our attention on the requirements of a support surface to produce the desired objectives. The ideal physical properties of the matrix, the properties of the introduced reactive groups, the best surface activation degree to reach the desired objective, and the properties of the reactive groups will be discussed. | Versión del editor: | https://doi.org/10.1002/cctc.201500310 | URI: | http://hdl.handle.net/10261/190645 | DOI: | 10.1002/cctc.201500310 | ISSN: | 1867-3880 | E-ISSN: | 1867-3899 |
Aparece en las colecciones: | (ICP) Artículos |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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