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Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination

AuthorsNg, Joseph D.; Gavira Gallardo, J. A. ; García Ruiz, Juan Manuel
KeywordsProtein crystallization
High throughput
Crystallization cassette
Issue DateApr-2003
CitationJournal of Structural Biology 142(1): 218-231 (2003)
AbstractCounterdiffusion crystallization in capillary is a very simple, cost-effective, and practical procedure for obtaining protein crystals suitable for X-ray data analysis. Its principles have been derived using well-known concepts coupling the ideas of precipitation and diffusion mass transport in a restricted geometry. The counterdiffusion process has been used to simultaneously screen for optimal conditions for protein crystal growth, incorporate strong anomalous scattering atoms, and mix in cryogenic solutions in a single capillary tube. The crystals obtained in the capillary have been used in situ for X-ray analysis. The implementation of this technique linked to the advancement of current crystallography software leads to a powerful structure determination method consolidating crystal growth, X-ray data collection, and ab initio phase determination into one without crystal manipulation. We review the historical progress of counterdiffusion crystallization, its application to X-ray crystallography, and ongoing tool development for high-throughput protein structure determination.
Description14 pages, 9 figures, 2 tables.
Publisher version (URL)http://dx.doi.org/10.1016/S1047-8477(03)00052-2
Appears in Collections:(IACT) Artículos
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