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dc.contributor.authorGarrido, Diegoes_ES
dc.contributor.authorKabeya, Naokies_ES
dc.contributor.authorHontoria, Franciscoes_ES
dc.contributor.authorNavarro, Juan Carloses_ES
dc.contributor.authorReis, D. B.es_ES
dc.contributor.authorMartín, Virginiaes_ES
dc.contributor.authorRodríguez, Covadongaes_ES
dc.contributor.authorAlmansa, E.es_ES
dc.contributor.authorMonroig, Óscares_ES
dc.date.accessioned2019-09-11T11:22:01Z-
dc.date.available2019-09-11T11:22:01Z-
dc.date.issued2019-08-
dc.identifier.citationBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1864(8): 1134-1144 (2019)es_ES
dc.identifier.issn0006-3002-
dc.identifier.urihttp://hdl.handle.net/10261/190478-
dc.description.abstractThe interest in understanding the capacity of aquatic invertebrates to biosynthesise omega-3 (ω3) long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) has increased in recent years. Using the common octopus Octopus vulgaris as a model species, we previously characterised a ∆5 desaturase and two elongases (i.e. Elovl2/5 and Elovl4) involved in the biosynthesis of LC-PUFA in molluscs. The aim of this study was to characterise both molecularly and functionally, two methyl-end (or ωx) desaturases that have been long regarded to be absent in most animals. O. vulgaris possess two ωx desaturase genes encoding enzymes with ∆12 and ω3 regioselectivities enabling the de novo biosynthesis of the C18 PUFA 18:2ω6 (LA, linoleic acid) and 18:3ω3 (ALA, α-linolenic acid), generally regarded as dietary essential for animals. The O. vulgaris ∆12 desaturase (“ωx2”) mediates the conversion of 18:1ω9 (oleic acid) into LA, and subsequently, the ω3 desaturase (“ωx1”) catalyses the ∆15 desaturation from LA to ALA. Additionally, the O. vulgaris ω3 desaturase has ∆17 capacity towards a variety of C20 ω6 PUFA that are converted to their ω3 PUFA products. Particularly relevant was the affinity of the ω3 desaturase towards 20:4ω6 (ARA, arachidonic acid) to produce 20:5ω3 (EPA, eicosapentaenoic acid), as supported by yeast heterologous expression, and enzymatic activity exhibited in vivo when paralarvae were incubated in the presence of [1-14C]20:4ω6. These results confirmed that several routes enabling EPA biosynthesis are operative in O. vulgaris whereas ARA and docosahexaenoic acid (DHA, 22:6ω3) should be considerees_ES
dc.description.sponsorshipThis study was funded by the Ministerio de Ciencia e Innovación (Spanish Government) under Project OCTOWELF (Ref. AGL2013-49101-C2-1-R). The authors wish to thank the Instituto Español de Oceanografía for the grant FPI 2011 “Biomarcadores de estrés y metabolismo lipídico en las primeras fases de vida del pulpo común (Octopus vulgaris)” (BOE of 3rd November 2011), as well as COST Action FA1301 (Ref. COST-STSM-ECOST-STSM-FA1301-030815-063868) by funding DG during his visit at Institute of Aquaculture, University of Stirling (Scotland, UK). Further funding was obtained through a Proyecto Intramural Especial of CSIC (201840I016) awarded to ÓM. CR is a member of the Instituto de Biotecnología de Canarias (ITB).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/AGL2013-49101-C2-1-Res_ES
dc.relation.isversionofPostprint-
dc.rightsembargoedAccesses_ES
dc.subjectBiosynthesises_ES
dc.subjectEssential fatty acidses_ES
dc.subjectMethyl-end desaturaseses_ES
dc.subjectOctopus vulgarises_ES
dc.titleMethyl-end desaturases with ∆12 and ω3 regioselectivities enable the de novo PUFA biosynthesis in the cephalopod Octopus vulgarises_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.bbalip.2019.04.012-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.bbalip.2019.04.012es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderInstituto Español de Oceanografíaes_ES
dc.contributor.funderUniversity of Stirlinges_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011034es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002326es_ES
dc.contributor.orcidGarrido, Diego [0000-0002-1592-4877]es_ES
dc.contributor.orcidKabeya, Naoki [0000-0002-2055-6554]es_ES
dc.contributor.orcidHontoria, Francisco [0000-0003-2466-1375]es_ES
dc.contributor.orcidNavarro, Juan Carlos [0000-0001-6976-6686]es_ES
dc.contributor.orcidMonroig, Óscar [0000-0001-8712-0440]es_ES
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