Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/190478
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Methyl-end desaturases with ∆12 and ω3 regioselectivities enable the de novo PUFA biosynthesis in the cephalopod Octopus vulgaris |
Autor: | Garrido, Diego; Kabeya, Naoki; Hontoria, Francisco CSIC ORCID ; Navarro, Juan Carlos CSIC ORCID ; Reis, Diana B.; Martín, Virginia; Rodríguez, Covadonga; Almansa, E.; Monroig, Óscar CSIC ORCID | Palabras clave: | Biosynthesis Essential fatty acids Methyl-end desaturases Octopus vulgaris |
Fecha de publicación: | ago-2019 | Editor: | Elsevier | Citación: | Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1864(8): 1134-1144 (2019) | Resumen: | The interest in understanding the capacity of aquatic invertebrates to biosynthesise omega-3 (ω3) long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) has increased in recent years. Using the common octopus Octopus vulgaris as a model species, we previously characterised a ∆5 desaturase and two elongases (i.e. Elovl2/5 and Elovl4) involved in the biosynthesis of LC-PUFA in molluscs. The aim of this study was to characterise both molecularly and functionally, two methyl-end (or ωx) desaturases that have been long regarded to be absent in most animals. O. vulgaris possess two ωx desaturase genes encoding enzymes with ∆12 and ω3 regioselectivities enabling the de novo biosynthesis of the C18 PUFA 18:2ω6 (LA, linoleic acid) and 18:3ω3 (ALA, α-linolenic acid), generally regarded as dietary essential for animals. The O. vulgaris ∆12 desaturase (“ωx2”) mediates the conversion of 18:1ω9 (oleic acid) into LA, and subsequently, the ω3 desaturase (“ωx1”) catalyses the ∆15 desaturation from LA to ALA. Additionally, the O. vulgaris ω3 desaturase has ∆17 capacity towards a variety of C20 ω6 PUFA that are converted to their ω3 PUFA products. Particularly relevant was the affinity of the ω3 desaturase towards 20:4ω6 (ARA, arachidonic acid) to produce 20:5ω3 (EPA, eicosapentaenoic acid), as supported by yeast heterologous expression, and enzymatic activity exhibited in vivo when paralarvae were incubated in the presence of [1-14C]20:4ω6. These results confirmed that several routes enabling EPA biosynthesis are operative in O. vulgaris whereas ARA and docosahexaenoic acid (DHA, 22:6ω3) should be considere | Versión del editor: | http://dx.doi.org/10.1016/j.bbalip.2019.04.012 | URI: | http://hdl.handle.net/10261/190478 | DOI: | 10.1016/j.bbalip.2019.04.012 | ISSN: | 0006-3002 |
Aparece en las colecciones: | (IATS) Artículos (IEO) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Methyl-end_desaturases.pdf | 2,37 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
19
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
17
checked on 27-feb-2024
Page view(s)
300
checked on 24-abr-2024
Download(s)
304
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.