English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/190352
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

AmrZ and FleQ co-regulate cellulose production in pseudomonas syringae pv. Tomato DC3000

AuthorsPérez-Mendoza, Daniel; Felipe, Antonia; Ferreiro, María Dolores; Sanjuán, Juan; Gallegos, María Trinidad
KeywordsTranscriptional regulation, Pseudomonas syringae
C-di-GMP
Cellulose
FleQ, AmrZ
Issue Date2019
PublisherFrontiers Media
CitationFrontiers in Microbiology 10: 746 (2019)
AbstractPseudomonas syringae pv. tomato DC3000 carries the wssABCDEFGHI operon for the synthesis of acetylated cellulose, whose production is stimulated by increasing the intracellular levels of the second messenger c-di-GMP. This enhances air-liquid biofilm formation and generates a wrinkly colony morphotype in solid media. In the present study we show that cellulose production is a complex process regulated at multiple levels and involving different players in this bacterium. Using different in vitro approaches, including Electrophoretic Mobility Shift Assay (EMSA) and footprint analysis, we demonstrated the interrelated role of two transcriptional regulators, AmrZ and FleQ, over cellulose production in Pto DC3000 and the influence of c-di-GMP in this process. Under physiological c-di-GMP levels, both regulators bind directly to adjacent regions at the wss promoter inhibiting its expression. However, just FleQ responds to c-di-GMP releasing from its wss operator site and converting from a repressor to an activator of cellulose production. The additive effect of the double amrZ/fleQ mutation on the expression of wss, together with the fact that they are not cross-regulated at the transcriptional level, suggest that FleQ and AmrZ behave as independent regulators, unlike what has been described in other Pseudomonas species. Furthermore, this dual co-regulation exerted by AmrZ and FleQ is not limited to cellulose production, but also affects other important phenotypes in Pto DC3000, such as motility and virulence.
Publisher version (URL)https://www.frontiersin.org/articles/10.3389/fmicb.2019.00746/full
URIhttp://hdl.handle.net/10261/190352
DOI10.3389/fmicb.2019.00746
Identifiersdoi: 10.3389/fmicb.2019.00746
issn: 1664-302X
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
2019_PerezMendoza_FM_OA.pdf4,47 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.