English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/189968
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Carp Il10a and Il10b exert identical biological activities in vitro, but are differentially regulated in vivo

AuthorsPiazzon de Haro, María Carla ; Wentzel, Annelieke S.; Wiegertjes, Geert F.
KeywordsInterleukin-10
Teleost
Paralogues
Gene duplication
Issue DateFeb-2017
PublisherElsevier
CitationDevelopmental and Comparative Immunology 67: 350-360 (2017)
AbstractWe recently reported on the functional characterization of carp Il10. We showed that carp Il10 is able to downregulate proinflammatory activities by carp phagocytes and promote B cell proliferation, differentiation and antibody production as well as proliferation of memory T cells. Taking advantage of the recent annotation of the carp genome, we completed the sequence of a second il10 paralogue, named il10b, the presence of which was expected owing to the recent (8 million years ago) fourth round of whole genome duplication that occurred in common carp. In the present study we closely compared the two Il10 paralogues and show that Il10a and Il10b have almost identical gene structure, synteny, protein sequence as well as bioactivity on phagocytes. Although the two il10 paralogues show a large overlap in tissue expression, il10b has a low constitutive expression and is highly upregulated upon infection, whereas il10a is higher expressed under basal conditions but its gene expression remains constant during viral and parasitic infections. This differential regulation is most likely due to the observed differences in their promoter regions. Altogether our results demonstrate that gene duplication in carp, although recent, led to sub-functionalization and expression divergence rather than functional redundancy of the Il10 paralogues, yet with very similar protein sequences
Publisher version (URL)http://dx.doi.org/10.1016/j.dci.2016.08.016
URIhttp://hdl.handle.net/10261/189968
DOI10.1016/j.dci.2016.08.016
ISSN0145-305X
E-ISSN1879-0089
Appears in Collections:(IATS) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,35 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.