Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/18959
Título : New applications of light and electron microscopic techniques for the study of microbiological inclusion in amber
Autor : Ascaso, Carmen, Wierzchos, Jacek, Corral, J. Carmelo, López, Rafael, Alonso, Jesús
Palabras clave : Ámbar
Microscopia confocal
Álava
SEM
LT SEM
Crioscanning
Microbología de inclusión
Fecha de publicación : Nov-2003
Editor: Society of Economic Paleontologists and Mineralogists
Citación : Journal of Paleontology 77(6): 1182-1192 (2003)
Resumen: Amber is a superb medium for the fossilization of delicate organisms. Besides light microscopy techniques for the study of insects in amber, scanning electron microscopy (SEM) in backscattered electron mode (SEM-BSE), low temperature SEM (LTSEM) and also confocal laser scanning microscopy (CLSM) were used to examine microcenosis and particulate plant remains (microdebris). We applied these techniques to such inclusions in amber A´ lava, northern Spain (Allaian: Early Cretaceous). Confocal microscopy provides a 3D image of partial microcenosis showing bifurcate fungal hyphae. The huge potential of SEM-BSE yields high resolution images, in which the relationship between protozoa and fungal hyphae may be observed and the characterization of further ultrastructural details in flagellates. According to the SEM-BSE images, food and pulsatile vacuoles appear better preserved than mitochondria and lipids in amber-embedded protozoa. A process of protozoan mineralization has led to the deposition of S and Fe in peripheral areas, and the Fe is also present in the core of surrounding fungal hyphae. Application of LTSEM for the study of protozoan inclusions produces images of their exteriors showing many vacuoles. Plant tissues under SEM-BSE show mummified cell walls, while the cytoplasm exhibits a bright appearance and is very rich in Fe and S. SEM in secondary electron mode (SEM-SE) also reveals a microbiota trapped in gas bubbles.
Descripción : 11 pages, 5 figures
URI : http://hdl.handle.net/10261/18959
ISSN: 0022-3360
Citación : Journal of Paleontology 77(6): 1182-1192 (2003)
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