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Collection device for keeping microbiota viability

AuthorsMartínez Álvarez, Noelia ; Hidalgo-Cantabrana, Claudio ; Margolles Barros, Abelardo ; Delgado, Susana ; Sánchez García, Borja
Issue Date26-Jun-2018
Citation7th IHMC (2018)
AbstractNew studies and therapies based on gut microbiota sampling need from protocol normalization and standardization. During this process, keeping microbial viability from the sampling to the delivery, and the manipulation and storage of the faecal samples is crucial. This standardization requires a device/kit to avoid the toxic effect of oxygen on intestinal microbiota. [Objective] To develop and validate an anaerobic stool sample collection device: GutAliveR (PCT/ES2017/070087). [Methods] The efficiency of GutAliveRwas tested and compared with other stool collection systems. Maintenance of the anaerobic conditions was experimentally evaluated by means of total counts of anaerobic microorganisms. For this purpose, autoclaved faecal samples were inoculated with cultures of different anaerobic microorganisms. Also, a biodiversity index was calculated from faecal samples of healthy donors. Recounts were carried out at inoculation time and after 5/24/72 h incubation at room temperature (RT). [Results] In GutAliveR , anaerobic microorganisms inoculated showed a lower loss of viability after 24 and 72 h compared with conventional systems. Bifidobacterium bifidum remained viable and culturable up to 72 h at RT, while using a conventional system the bifidobacterial population was reduced 37% at 24 h and more than 99% at 72 h. The biodiversity index is higher when GutAliveR is used to collect and transport the faecal samples. [Conclusion] GutAliveR ensures an anoxic environment for the stool sampling and transport until processing at the laboratory. This could be critical in some applications, such as standardization and normalization of microbiota-based studies and the isolation of new probiotic strains from faeces.
DescriptionTrabajo presentado en el 7th International Human Microbiome Congress, IHMC, celebrado en County Kerry (Irlanda) del 28 al 26 de junio de 2018
Appears in Collections:(IPLA) Comunicaciones congresos
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