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Identification of S-alleles in almond using multiplex PCR

AuthorsSánchez-Pérez, Raquel ; Dicenta, Federico ; Martínez-Gómez, Pedro
Breeding program
Marker-assisted selection
Molecular markers
Issue DateAug-2004
CitationEuphytica 138(3): 263-269 (2004)
AbstractThe S-genotypes of eight almond (Prunus dulcis Miller (D.A. Webb)) cultivars from different geographical origins and of nine new selections from the CEBAS-CSIC (Murcia, Spain) breeding program were determined using single and multiplex PCR with different sets of specific oligonucleotide primers. The results of PCR using the AS1II- and AmyC5R-specific primers showed amplification in a single reaction of 10 different self-incompatibility alleles and of the self-compatibility allele S f. However, the amplified fragments of the S f allele were of similar sizes to those amplified from the S 3 self-incompatibility allele. For this reason, a specific PCR primer CEBASf was designed from the intron sequence of S f. A multiplex-PCR reaction using the AS1II, CEBASf and AmyC5R primers permitted unequivocal identification of the 10 self-incompatibility alleles and of the self-compatibility allele. Multiplex PCR opens the possibility to identify new S-alleles using different sets of primers. The applications of these PCR markers in the almond-breeding programs are discussed.
Description7 pages, 3 figures, 2 tables.-- Subject Collection Biomedical and Life Sciences.
Publisher version (URL)http://dx.doi.org/10.1023/B:EUPH.0000047097.96271.bf
Appears in Collections:(CEBAS) Artículos
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