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Open Access item Comparison of in vitro models to study bacterial adhesion to the intestinal epithelium

Authors:Laparra, José Moisés
Sanz, Yolanda
Keywords:Adhesion, Caco-2, HT29-MTX, Intestinal, Mucin, Probiotics
Issue Date:25-Aug-2009
Publisher:Wiley-Blackwell
Citation:Letters in Applied Microbiology 49 (6): 695-701 (2009)
Abstract:[Aims]: To evaluate the adhesion ability of intestinal bacteria to different in vitro models of intestinal epithelia, and to estimate the suitability of these models and the type of interactions involved.
[Methods and results]: The adhesion of probiotic (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12), commensal (B. animalis IATA-A2 and B. bifidum IATA-ES2) and potentially pathogenic bacteria (E. coli and L. monocytogenes) was determined. The adhesion models used were polycarbonate-well plates, with or without mucin, and different configurations of Caco-2 and/or HT29-MTX cell cultures. All bacteria adhered to wells without mucin (2·6–27·3%), the values being highly variable depending on the bacterial strain. Adhesion percentages of potentially probiotic bacteria to Caco-2 cultures were remarkably lower (P < 0·05) than those to mucin, and more similar to those of pathogenic strains. The lowest adhesion of different bacterial strains was detected on HT29-MTX (0·5–2·3%) cultures and Caco-2/HT29-MTX (0·6–3·2%) cocultures, while these values were increased in Caco-2 cultures plus mucin.
[Conclusions]: The results suggested that bacterial strains exhibit different capacities to adhere to cellular components and several types of mucin present in different models, showing preferences for intestinal MUC2.
[Significance and impact of the study]: The use of Caco-2 cells monolayer plus mucin (type II) better approaches the physiological characteristics of in vivo situation, providing a reliable and suitable in vitro model to evaluate bacterial adhesion.
Description:7 pags, 1 table.-- Printed version published December 2009.-- The definitive version is available at www3.interscience.wiley.com
Publisher version (URL):http://dx.doi.org/10.1111/j.1472-765X.2009.02729.x
URI:http://hdl.handle.net/10261/18748
ISSN:0266-8254 (print)
1472-765X (online)
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Appears in Collections:(IATA) Artículos

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