English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/18737
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Improved technique for counting chromosomes in almond

AuthorsMartínez-Gómez, Pedro ; Sánchez-Pérez, Raquel ; Vaknin, Y.; Dicenta, Federico ; Gradziel, Thomas M.
KeywordsAlmond
Prunus dulcis
Mitosis
Chromosomes
Issue Date30-May-2005
PublisherElsevier
CitationScientia Horticulturae 105(1): 139-143 (2005)
AbstractIn almond [Prunus dulcis (Mill.) D.A. Webb], there are few chromosome studies. In this work, different methods described for slide preparation and staining of chromosomes in other Prunus species have been evaluated using almond root tips. By modifying and updating traditional methods, an improved technique for counting chromosomes has been developed. Root tips were first placed in cold water for 4 h at 0 °C and treated with 0.2% colchicine for 3 h at 5 °C. They were then fixed for 24 h at 5 °C in methanol, propionic acid, chloroform (6:3:2), and stored in 70% ethanol. This optimized protocol allowed the identification of chromosomes in the different stages of mitosis late prophase being the best stage for chromosome counting.
Description5 pages, 1 figure.-- Short communication.
Publisher version (URL)http://dx.doi.org/10.1016/j.scienta.2005.01.019
URIhttp://hdl.handle.net/10261/18737
DOI10.1016/j.scienta.2005.01.019
ISSN0304-4238
Appears in Collections:(CEBAS) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.