English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/187291
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos

AuthorsMartínez, Rubén ; Herrero-Nogareda, Laia; Van Antro, Morgane; Campos, María Pilar; Casado, Marta ; Barata Martí, Carlos ; Piña, Benjamín ; Navarro-Martín, Laia
Endocrine disruption
Issue Date21-Jun-2019
CitationAquatic Toxicology 214: 105232 (2019)
AbstractUnderstanding the mode of action of the different pollutants in human and wildlife health is a key step in environmental risk assessment. The aim of this study was to determine signatures that could link morphological phenotypes to the toxicity mechanisms of four Endocrine Disrupting Chemicals (EDCs): bisphenol A (BPA), perfluorooctanesulfonate potassium salt (PFOS), tributyltin chloride (TBT), and 17-ß-estradiol (E2). Zebrafish (Danio rerio) eleutheroembryos were exposed from 2 to 5 dpf to a wide range of BPA, PFOS, TBT and E2 concentrations. At the end of the exposures several morphometric features were assessed. Common and non-specific effects on larvae pigmentation or swim bladder area were observed after exposures to all compounds. BPA specifically induced yolk sac malabsorption syndrome and altered craniofacial parameters, whereas PFOS had specific effects on the notochord formation presenting higher rates of scoliosis and kyphosis. The main effect of E2 was an increase in the body length of the exposed eleutheroembryos. In the case of TBT, main alterations on the morphological traits were related to developmental delays. When integrating all morphometrical parameters, BPA showed the highest rates of malformations in terms of equilethality, followed by PFOS and, distantly, by TBT and E2. In the case of BPA and PFOS, we were able to relate our results with effects on the transcriptome and metabolome, previously reported. We propose that methodized morphometric analyses in zebrafish embryo model can be used as an inexpensive and easy screening tool to predict modes of action of a wide-range number of contaminants.
Publisher version (URL)https://doi.org/10.1016/j.aquatox.2019.105232
Appears in Collections:(IDAEA) Artículos
Files in This Item:
File Description SizeFormat 
Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish.pdf Embargoed until June 13, 2021Artículo principal856,25 kBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.