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Title

Capillary Crystallization and molecular replacement solution of Haemoglobin II of the Clam Lucina pectinata

AuthorsGavira Gallardo, J. A. ; Jesús, W. de; Cámara-Artigas, A.; López-Garriga, J.; García Ruiz, Juan Manuel
KeywordsHaemoglobin II
Lucina pectinata
Issue DateMar-2006
PublisherInternational Union of Crystallography
CitationActa Crystallographica Section F 62(3): 196-199 (2006)
AbstractHaemoglobin II is one of three haemoglobins present in the cytoplasm of the Lucina pectinata mollusc that inhabits the Caribbean coast. Using HBII purified from its natural source, crystallization screening was performed using the counter-diffusion method with capillaries of 0.2 mm inner diameter. Crystals of HbII suitable for data collection and structure determination were grown in the presence of agarose at 0.1%(w/v) in order to improve their quality. The crystals belong to the tetragonal space group P42212, with unit-cell parameters a = b = 73.92, c = 152.35 Å, and diffracted X-rays to a resolution of better than 2.0 Å. The asymmetric unit is a homodimer with a corresponding Matthews coefficient (VM) of 3.15 Å3 Da-1 and a solvent content of 61% by volume.
Description4 pages, 3 figures, 1 table.
Publisher version (URL)http://dx.doi.org/10.1107/S1744309106002648
URIhttp://hdl.handle.net/10261/18706
DOI10.1107/S1744309106002648
ISSN1067-0696
Appears in Collections:(IACT) Artículos
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