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Título: | Carrier-free immobilization of lipase from candida rugosa with polyethyleneimines by carboxyl-activated cross-linking |
Autor: | Velasco-Lozano, Susana CSIC ORCID; López-Gallego, Fernando CSIC ORCID; Vazquez-Duhalt, Rafael; Mateos-Díaz, Juan C.; Guisán, José Manuel CSIC ORCID ; Favela-Torres, Ernesto | Fecha de publicación: | 12-may-2014 | Editor: | American Chemical Society | Citación: | Biomacromolecules 15(5): 1896-1903 (2014) | Resumen: | Carrier-free immobilization of Candida rugosa lipase (CRL) and polymers containing primary amino groups were cross-linked using carbodiimide. To accomplish this, the free carboxyl groups of the enzyme were activated with carbodiimide-succinimide in organic medium, and then the activated proteins were cross-linked with different polyethylenimines (PEIs). The effect of the cross-linker chain length, the amount of added bovine serum albumin (BSA), and carbodiimide concentration on the catalytic properties of resulting cross-linked enzyme aggregates (CLEAs) was investigated. The CLEAs’ size, shape, specific activity, activity recovery, thermostability and enantioselectivity significantly varied according to the preparation procedure. The highest thermostable CRL-CLEA preparation was obtained with 1.3 kDa polyethyleneimine as cross-linker, 10 mg of BSA and 28 mM of carbodiimide. This preparation is 1.3-fold more active and thermostable than CLEAs prepared by the traditional method of amino cross-linking with glutaraldehyde, and retains 60% of residual activity after 22 h at 50 °C. Additionally, the CRL-CLEA preparation showed an enantioselectivity of 91% enantiomeric excess (ee). This immobilization procedure provides an alternative strategy for CLEA production, particularly for enzymes where the traditional method of cross-linking via lysine residues leads to enzyme inactivation. | Versión del editor: | https://doi.org/10.1021/bm500333v | URI: | http://hdl.handle.net/10261/186192 | DOI: | 10.1021/bm500333v | ISSN: | 1525-7797 | E-ISSN: | 1526-4602 |
Aparece en las colecciones: | (ICP) Artículos |
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