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dc.contributor.authorMartínez Álvarez, Noeliaes_ES
dc.contributor.authorLuque, Robertoes_ES
dc.contributor.authorMilani, Christianes_ES
dc.contributor.authorVentura, Marcoes_ES
dc.contributor.authorBañuelos, Oscares_ES
dc.contributor.authorMargolles Barros, Abelardoes_ES
dc.date.accessioned2019-07-10T12:32:39Z-
dc.date.available2019-07-10T12:32:39Z-
dc.date.issued2018-05-
dc.identifier.citationApplied and Environmental Microbiology 84(10): e02888-17 (2017)es_ES
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/185858-
dc.description.abstractBifidobacteria are mutualistic intestinal bacteria, and their presence in the human gut has been associated with health-promoting activities. The presence of antibiotic resistance genes in this genus is controversial, since, although bifidobacteria are nonpathogenic microorganisms, they could serve as reservoirs of resistance determinants for intestinal pathogens. However, until now, few antibiotic resistance determinants have been functionally characterized in this genus. In this work, we show that Bifidobacterium breve CECT7263 displays atypical resistance to erythromycin and clindamycin. In order to delimit the genomic region responsible for the observed resistance phenotype, a library of genomic DNA was constructed and a fragment of 5.8 kb containing a gene homologous to rRNA methylase genes was able to confer erythromycin resistance in Escherichia coli. This genomic region seems to be very uncommon, and homologs of the gene have been detected in only one strain of Bifidobacterium longum and two other strains of B. breve. In this context, analysis of shotgun metagenomics data sets revealed that the gene is also uncommon in the microbiomes of adults and infants. The structural gene and its upstream region were cloned into a B. breve-sensitive strain, which became resistant after acquiring the genetic material. In vitro conjugation experiments did not allow us to detect gene transfer to other recipients. Nevertheless, prediction of genes potentially acquired through horizontal gene transfer events revealed that the gene is located in a putative genomic island. IMPORTANCE Bifidobacterium breve is a very common human intestinal bacterium. Often described as a pioneer microorganism in the establishment of early-life intestinal microbiota, its presence has been associated with several beneficial effects for the host, including immune stimulation and protection against infections. Therefore, some strains of this species are considered probiotics. In relation to this, because probiotic bacteria are used for human and animal consumption, one of the safety concerns over these bacteria is the presence of antibiotic resistance genes, since the human gut is a densely populated habitat that could favor the transfer of genetic material to potential pathogens. In this study, we analyzed the genetic basis responsible for the erythromycin and clindamycin resistance phenotype of B. breve CECT7263. We were able to identify and characterize a novel gene homologous to rRNA methylase genes which confers erythromycin and clindamycin resistance. This gene seems to be very uncommon in other bifidobacteria and in the gut microbiomes of both adults and infants. Even though conjugation experiments showed the absence of transferability under in vitro conditions, it has been predicted to be located in a putative genomic island recently acquired by specific bifidobacterial strains.es_ES
dc.description.sponsorshipThis research was carried out thanks to the grant METASIN (Investigación, Desarrollo e Innovación en Nuevos Alimentos Multifuncionales para Síndrome Metabolic), funded by the Centro Para el Desarrollo Tecnológico Industrial (CDTI) and Biosearch S.A. This work was also funded by the EU Joint Programming Initiative A Healthy Diet for a Healthy Life (JPI HDHL, http://www.healthydietforhealthylife.eu/) and MIUR to M.V. This research benefits from the HPC (High-Performance Computing) facility of the University of Parma, Parma, Italy.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.rightsclosedAccesses_ES
dc.subjectBifidobacteriumes_ES
dc.subjectBifidobacterium brevees_ES
dc.subjectClindamycin resistancees_ES
dc.subjectErythromycin resistancees_ES
dc.subjectMicrobiotaes_ES
dc.subjectProbioticses_ES
dc.titleA gene homologous to rRNA methylase genes confers erythromycin and clindamycin resistance in Bifidobacterium brevees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1128/AEM.02888-17-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1128/AEM.02888-17es_ES
dc.identifier.e-issn1098-5336-
dc.contributor.funderCentro para el Desarrollo Tecnológico Industrial (España)-
dc.contributor.funderEuropean Commission-
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001872es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.contributor.orcidMilani, Christian [0000-0002-5062-3164]es_ES
dc.contributor.orcidVentura, Marco [0000-0002-4875-4560]es_ES
dc.contributor.orcidMargolles Barros, Abelardo [0000-0003-2278-1816]es_ES
dc.identifier.pmid29500262-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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