English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/185106
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

The biosynthesis of flavin cofactors in Listeria monocytogenes

AuthorsSebastián Valverde, María; Arilla-Luna, Sonia; Bellalou, Jacques; Yruela Guerrero, Inmaculada ; Medina Trullenque, Milagros
KeywordsFMN and FAD biosynthesis
riboflavin kinase
FMN:ATP adenylyltransferase
FAD synthase
Listeria monocytogenes
Issue DateJul-2019
CitationSebastián M, Arilla-Luna S, Bellalou J, Yruela I, Medina M. The biosynthesis of flavin cofactors in Listeria monocytogenes. Journal of Molecular Biology 431 (15): 2762-2776 (2019)
AbstractListeria monocytogenes is riboflavin auxotrophic, but it has two genes envisaged to transform riboflavin into FMN and FAD after its uptaked by specialized transporters. One encodes a bifunctional type I FAD synthase (FADS, herein LmFADS-1), while the other produces a protein similar to type I at the FMN:ATP adenylyltransferase (FMNAT) site but with a shorter C-terminal that lacks any riboflavin kinase (RFK) motif. This second protein is rare among bacteria and has been named FADS type II (LmFADS-2). Here we present a biochemical and biophysical study of LmFADS-1 and LmFADS-2 by integrating kinetic and thermodynamic data together with sequence and structural prediction methods to evaluate their occurrence in Listeria, as well as their function and molecular properties. Despite LmFADS-1 similarities to other type I FADSs, (i) its RFK activity has not riboflavin substrate inhibition and occurs under reducing and oxidizing conditions, (ii) its FMNAT activity requires strong reducing environment, and (iii) binding of reaction products, but not substrates, favors binding of the second ligand. LmFADS-2 produces FAD under oxidizing and reducing environments, but its C-terminus module function remains unknown. Listeria species conserve both FADSs, being sequence identity high within L. monocytogenes strains. Our data exemplify alternative strategies for FMN and FAD biosynthesis and homeostasis, envisaging that in Listeria two FADSs might be required to fulfill the supply of flavin cofactors under niches that can go from saprophytism to virulence. As FADSs are attractive antimicrobial targets, understanding of FADSs traits in different species is essential to help in the discovery of specific antimicrobials.
Description55 Pags.- 7 Figs.- Suppl. Mat. (9 Figs.- 5 Tabls.). The definitive version is available at: https://www.sciencedirect.com/science/journal/00222836
Publisher version (URL)https://doi.org/10.1016/j.jmb.2019.05.029
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
YruelaI_J-MolBiol_2019.pdf Embargoed until August 1, 20213 MBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.