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Título: | Identification and expression of the 11b-steroid hydroxylase from Cochliobolus lunatus in Corynebacterium glutamicum |
Autor: | Felpeto-Santero, Carmen CSIC; Galán, Beatriz CSIC ORCID ; Luengo, José M.; Fernández-Cañón, José Manuel; Del Cerro, Carlos CSIC ORCID; Medrano, Francisco Javier CSIC ORCID ; García, José Luis CSIC ORCID | Fecha de publicación: | 14-jun-2019 | Editor: | John Wiley & Sons | Citación: | Microb Biotechnol 12(5): 856–868 (2019) | Resumen: | Hydroxylation of steroids has acquired special relevance for the pharmaceutical industries. Particularly, the 11β-hydroxylation of steroids is a reaction of biotechnological importance currently carried out at industrial scale by the fungus Cochliobolus lunatus. In this work, we have identified the genes encoding the cytochrome CYP103168 and the reductase CPR64795 of C. lunatus responsible for the 11β-hydroxylase activity in this fungus, which is the key step for the preparative synthesis of cortisol in industry. A recombinant Corynebacterium glutamicum strain harbouring a plasmid expressing both genes forming a synthetic bacterial operon was able to 11β-hydroxylate several steroids as substrates. This is a new example to show that the industrial strain C. glutamicum can be used as a suitable chassis to perform steroid biotransformation expressing eukaryotic cytochromes. | Versión del editor: | https://doi.org/10.1111/1751-7915.13428 | URI: | http://hdl.handle.net/10261/184623 | DOI: | 10.1111/1751-7915.13428 | E-ISSN: | 1751-7915 |
Aparece en las colecciones: | (CIB) Artículos |
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Microbial Biotechnology_Felpeto_2019.pdf | Artículo principal | 603,26 kB | Adobe PDF | Visualizar/Abrir |
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