English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/184495
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Molecular epidemiology and whole genome sequencing analysis of clinical Mycobacterium bovis from Ghana

AuthorsDarko Otchere, Isaac; Tonder, Andries J. van; Asante-Poku, Adwoa; Sánchez-Busó, Leonor; Coscollá, Mireia; Osei-Wusu, Stephen; Asare, Prince; Yaw Aboagye, Samuel; Acquah Ekuban, Samuel; Iddrisu Yahayah, Abdallah; Forson, Audrey; Baddoo, Akosua; Laryea, Clement; Parkhill, Julian; Harris, Simon R.; Gagneux, Sebastien; Yeboah-Manu, Dorothy
Issue Date2019
PublisherPublic Library of Science
CitationPLoS ONE 14(3): e0209395 (2019)
Abstract[Background]: Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. bovis strains with hyper-virulence, MDR-TB and disseminated disease makes it imperative to understand the biology of the pathogen.
[Methods]: Mycobacterium bovis (15) among 1755 M. tuberculosis complex (MTBC) isolated between 2012 and 2014 were characterized and analyzed for associated patient demography and other risk factors. Five of the M. bovis isolates were whole-genome sequenced and comparatively analyzed against a global collection of published M. bovis genomes.
[Results]: Mycobacterium bovis was isolated from 3/560(0.5%) females and 12/1195(1.0%) males with pulmonary TB. The average age of M. bovis infected cases was 46.8 years (7-72years). TB patients from the Northern region of Ghana (1.9%;4/212) had a higher rate of infection with M. bovis (OR = 2.7,p = 0.0968) compared to those from the Greater Accra region (0.7%;11/1543). Among TB patients with available HIV status, the odds of isolating M. bovis from HIV patients (2/119) was 3.3 higher relative to non-HIV patients (4/774). Direct contact with livestock or their unpasteurized products was significantly associated with bTB (p<0.0001, OR = 124.4,95% CI = 30.1–508.3). Two (13.3%) of the M. bovis isolates were INH resistant due to the S315T mutation in katG whereas one (6.7%) was RIF resistant with Q432P and I1491S mutations in rpoB. M. bovis from Ghana resolved as mono-phyletic branch among mostly M. bovis from Africa irrespective of the host and were closest to the root of the global M. bovis phylogeny. M. bovis-specific amino acid mutations were detected among MTBC core genes such as mce1A, mmpL1, pks6, phoT, pstB, glgP and Rv2955c. Additional mutations P6T in chaA, G187E in mgtC, T35A in Rv1979c, S387A in narK1, L400F in fas and A563T in eccA1 were restricted to the 5 clinical M. bovis from Ghana.
[Conclusion]: Our data indicate potential zoonotic transmission of bTB in Ghana and hence calls for intensified public education on bTB, especially among risk groups.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0209395
URIhttp://hdl.handle.net/10261/184495
DOI10.1371/journal.pone.0209395
E-ISSN1932-6203
Appears in Collections:(I2SysBio) Artículos
Files in This Item:
File Description SizeFormat 
moleculaGhana.pdf2,07 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.