Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/18394
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Pérez-Jiménez, Raúl | - |
dc.contributor.author | Kosuri, Pallav | - |
dc.contributor.author | Rodríguez-Larrea, David | - |
dc.contributor.author | Gavira Gallardo, J. A. | - |
dc.contributor.author | Fernández Soler, Julio M. | - |
dc.contributor.author | Sánchez-Ruiz, José M. | - |
dc.contributor.author | Wiita, Arun P. | - |
dc.date.accessioned | 2009-11-10T08:39:09Z | - |
dc.date.available | 2009-11-10T08:39:09Z | - |
dc.date.issued | 2008-10-03 | - |
dc.identifier.citation | Journal of Biological Chemistry 283(40): 27121-27129 (2008) | en_US |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/18394 | - |
dc.description | 22 pages, 6 figures, 1 table. | en_US |
dc.description.abstract | Understanding how the catalytic mechanisms of enzymes are optimized through evolution remains a major challenge in molecular biology. The concept of co-evolution implicates that compensatory mutations occur to preserve the structure and function of proteins. We have combined statistical analysis of protein sequences with the sensitivity of single molecule force-clamp spectroscopy to probe how catalysis is affected by structurally distant correlated mutations in Escherichia coli thioredoxin. Our findings show that evolutionary anti-correlated mutations have an inhibitory effect on enzyme catalysis, whereas positively correlated mutations rescue the catalytic activity. We interpret these results in terms of an evolutionary tuning of both the enzyme-substrate binding process and the chemistry of the active site. Our results constitute a direct observation of distant residue co-evolution in enzyme catalysis. | en_US |
dc.description.sponsorship | We thank Dr. Sergi Garcia-Manyes and Dr. Robert Szoszkiewicz for careful reading of the manuscript and all the Fernandez laboratory members for helpful discussions. | en_US |
dc.format.extent | 10752 bytes | - |
dc.format.mimetype | application/octet-stream | - |
dc.language.iso | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.rights | closedAccess | en_US |
dc.title | Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis. | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1074/jbc.M803746200 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.M803746200 | en_US |
dc.identifier.e-issn | 1083-351X | - |
dc.identifier.pmid | 18687682 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IACT) Artículos |
CORE Recommender
PubMed Central
Citations
8
checked on 10-abr-2024
SCOPUSTM
Citations
16
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 29-feb-2024
Page view(s)
366
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.