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Título: | Colocalization of estrogen receptors with the fluorescent tamoxifen derivative, FLTX1, analyzed by confocal microscopy |
Autor: | Morales, Araceli; Marín, Raquel CSIC; Marrero-Alonso, Jorge; Boto, Alicia CSIC ORCID ; Díaz, Mario | Palabras clave: | Fluorescent tamoxifen derivative Confocal microscopy FLTX1 Membrane estrogen receptors Intracellular estrogen receptors Estrogenreceptors |
Fecha de publicación: | 2016 | Editor: | Humana Press | Citación: | Methods in molecular biology 1366: 163-173 (2016) | Resumen: | Tamoxifen is a selective estrogen receptor modulator that competitively binds the ligand-binding domain of estrogen receptors. Binding of tamoxifen displaces its cognate ligand, 17β-estradiol, thereby hampering the activation of estrogen receptors. Cellular labeling of ER is typically carried out using specific antibodies which require permeabilization of cells, incubation with secondary antibodies, and are expensive and time consuming. In this article, we describe the usefulness of FLTX1, a novel fluorescent tamoxifen derivative, which allows the labeling of estrogen receptors in immunocytochemistry and immunohistochemistry studies, both under permeabilized and non-permeabilized conditions. Further, besides labeling canonical estrogen receptors, this novel fluorescent probe is also suitable for the identification of unconventional targets such membrane estrogen receptors as well as other noncanonical targets, some of which are likely responsible for the number of undesired side effects reported during long-term tamoxifen treatments. | Versión del editor: | https://doi.org/10.1007/978-1-4939-3127-9_13 | URI: | http://hdl.handle.net/10261/183939 | DOI: | 10.1007/978-1-4939-3127-9_13 | ISSN: | 1064-3745 | E-ISSN: | 1940-6029 |
Aparece en las colecciones: | (IPNA) Artículos |
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