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Title

Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution

AuthorsTorres‐Salas, Pamela; Maté, Diana M. ; Ghazi, Iraj ; Plou Gasca, Francisco José ; Ballesteros Olmo, Antonio ; Alcalde Galeote, Miguel
KeywordsLaccase
Saccharomyces cerevisiae
Enzymes
Directed evolution
C‐terminal plug
Alkaline pH
Issue Date27-May-2013
PublisherWiley-VCH
CitationChemistry: a European Journal 14(8): 934-937 (2013)
AbstractUnnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.
Publisher version (URL)https://doi.org/10.1002/cbic.201300102
URIhttp://hdl.handle.net/10261/183480
DOI10.1002/cbic.201300102
ISSN0947-6539
E-ISSN1521-3765
Appears in Collections:(ICP) Artículos
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