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Clickable magnetic nanoparticles - a new tool for magnetic hyperthermia

AuthorsFratila, Raluca M.; Navascuez, Marcos; Idiago López, Francisco Javier; Moreno Antolín, Eduardo; Eceiza, Maite; Aizpurua, Jesus M.; Fuente, Jesús M. de la
Issue Date2018
Citation12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers (2018)
AbstractMagnetic hyperthermia (MH), based on the ability of magnetic nanoparticles (MNPs) to generate heat when exposed to alternating magnetic fields (AMF) is a very active area of research, especially for the development of new therapeutic solutions for cancer treatment. Recently, our team has initiated a new research line focused on the use of bioorthogonal click chemistry for magnetic hyperthermia studies applied using MNPs covalently attached to cell membranes. Our interest lies in the sub-lethal version of MH, which we believe is a powerful tool to induce a controlled and localized heating of the cell membrane (“hotspots”). This could produce temporal changes in the membrane biophysical properties, which can be used for enhanced delivery of therapeutics. Bioorthogonal strain-promoted “click” [3 + 2] azide-alkyne cycloaddition (SPAAC) reaction uses the ring strain to activate the alkyne, thus avoiding the use of the cytotoxic Cu(I) catalyst typically employed for standard “click” azide-alkyne cycloadditions (CuAAC). Despite its numerous applications in biology and biochemistry, SPAAC is still relatively new for nanotechnology applications. Herein, we report a simple functionalization protocol to obtain water-soluble MNPs suitable for SPAAC click chemistry. Hydrophobic 12 nm iron oxide MNPs were synthesized following a seed-mediated thermal decomposition methodology and transferred to water by coating with a fluorescent amphiphilic polymer (poly(maleic anhydride-alt-1- octadecene), PMAO-TAMRA).5 The MNPs were further functionalized step-wise with polyethyleneglycol (PEG) or a glucopyranoside derivative (Glc) to increase colloidal stability and with a cyclooctynylamine derivative. We have tested their reactivity towards azide-functionalized surfaces, demonstrating their potential as bioorthogonal probes. We are currently working on the incorporation of the “clickable” MNPs on more complex substrates (azide-modified lipid bilayers as simplified models of animal cell membranes). Studies regarding the cytotoxicity of the MNPs and their covalent attachment on living cell membranes are also underway.
DescriptionResumen del trabajo presentado a la 12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, celebrada en Copenhague (Dinamarca) del 22 al 26 de mayo de 2018.
Appears in Collections:(ICMA) Comunicaciones congresos
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