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Title

Orientation and function of a membrane-bound enzyme monitored by electrochemical surface-enhanced infrared absorption spectroscopy

AuthorsGutiérrez-Sanz, Óscar ; Marqués, Marta; Pereira, Inês A. C.; Lacey, Antonio L. de; Lubitz, Wolfgang; Rüdiger Ortiz, Olaf
KeywordsSEIRA
Phospholipid bilayer membranes
Hydrogenase
Protein film electrochemistry
Issue Date5-Aug-2013
PublisherAmerican Chemical Society
CitationJournal of Physical Chemistry Letters 4(17): 2794- 2798 (2013)
AbstractElectrochemical SEIRA is the method of choice for characterizing a lipid-bilayer-anchored, membrane-bound hydrogenase immobilized on a gold electrode. This setup allows the study of the enzyme under conditions mimicking its natural environment. A single experiment provides all of the crucial spectroscopic information relating to the protein orientation, active site of the protein, and the lipid bilayer and also direct electrochemical determination of the catalytic H2 oxidation by the enzyme.
Publisher version (URL)https://doi.org/10.1021/jz4013678
URIhttp://hdl.handle.net/10261/183293
DOI10.1021/jz4013678
ISSN1948-7185
Appears in Collections:(ICP) Artículos
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