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Título: | Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast |
Autor: | Madrid, Marisa; Vázquez-Marín, Beatriz; Soto, Teresa; Franco, Alejandro; Gómez Gil, Elisa; Vicente, Jero; Gacto, Mariano; Pérez, Pilar CSIC ORCID; Cansado, José | Palabras clave: | MAPK PKC Rho (Rho GTPase) Schizosaccharomyces pombe Signal transduction Stress response |
Fecha de publicación: | 7-jul-2017 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 292: 11374-11387 (2017) | Resumen: | The two PKC orthologs Pck1 and Pck2 in the fission yeast Schizosaccharomyces pombe operate in a redundant fashion to control essential functions, including morphogenesis and cell wall biosynthesis, as well as the activity of the cell integrity pathway and its core element, the MAPK Pmk1. We show here that, despite the strong structural similarity and functional redundancy of these two enzymes, the mechanisms regulating their maturation, activation, and stabilization have a remarkably distinct biological impact on both kinases. We found that, in contrast to Pck2, putative in vivo phosphorylation of Pck1 within the conserved activation loop, turn, and hydrophobic motifs is essential for Pck1 stability and biological functions. Constitutive Pck activation promoted dephosphorylation and destabilization of Pck2, whereas it enhanced Pck1 levels to interfere with proper downstream signaling to the cell integrity pathway via Pck2. Importantly, although catalytic activity was essential for Pck1 function, Pck2 remained partially functional independent of its catalytic activity. Our findings suggest that early divergence from a common ancestor in fission yeast involved important changes in the mechanisms regulating catalytic activation and stability of PKC family members to allow for flexible and dynamic control of downstream functions, including MAPK signaling. | Versión del editor: | https://doi.org/10.1074/jbc.M117.786087 | URI: | http://hdl.handle.net/10261/183144 | DOI: | 10.1074/jbc.M117.786087 | ISSN: | 0021-9258 | E-ISSN: | 1083-351X |
Aparece en las colecciones: | (IBFG) Artículos |
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Differential functional regulation_Madrid.pdf | 1,88 MB | Adobe PDF | Visualizar/Abrir |
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