Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/183061
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Gutiérrez-Sanz, Óscar | - |
dc.contributor.author | Marques, Marta C. | - |
dc.contributor.author | Baltazar, Carla S. A. | - |
dc.contributor.author | Fernández, Víctor M. | - |
dc.contributor.author | Soares, Claudio M. | - |
dc.contributor.author | Pereira, Inês A. C. | - |
dc.contributor.author | López de Lacey, Antonio | - |
dc.date.accessioned | 2019-05-31T12:02:01Z | - |
dc.date.available | 2019-05-31T12:02:01Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | Journal of Biological Inorganic Chemistry 18(4): 419-427 (2013) | - |
dc.identifier.issn | 0949-8257 | - |
dc.identifier.uri | http://hdl.handle.net/10261/183061 | - |
dc.description.abstract | A combined experimental and theoretical study of the catalytic activity of a [NiFeSe] hydrogenase has been performed by H/D exchange mass spectrometry and molecular dynamics simulations. Hydrogenases are enzymes that catalyze the heterolytic cleavage or production of H2. The [NiFeSe] hydrogenases belong to a subgroup of the [NiFe] enzymes in which a selenocysteine is a ligand of the nickel atom in the active site instead of cysteine. The aim of this research is to determine how much the specific catalytic properties of this hydrogenase are influenced by the replacement of a sulfur by selenium in the coordination of the bimetallic active site versus the changes in the protein structure surrounding the active site. The pH dependence of the D2/H+ exchange activity and the high isotope effect observed in the Michaelis constant for the dihydrogen substrate and in the single exchange/double exchange ratio suggest that a “cage effect” due to the protein structure surrounding the active site is modulating the enzymatic catalysis. This “cage effect” is supported by molecular dynamics simulations of the diffusion of H2 and D2 from the outside to the inside of the protein, which show different accumulation of these substrates in a cavity next to the active site. | - |
dc.description.sponsorship | This work was funded by the research projects CTQ2009-12649 (Ministerio de Economía y Competitividad, MINECO, Spain), PEst-OE/EQB/LA0004/2011, BIA-MIC/104030/2008, and SFRH/BD/73369/2010 (Fundação para a Ciência e Tecnologia, Ministério da Educação e Ciência, Portugal), and by a Luso-Spanish Joint Action funded by Conselho de Reitores das Universidades Portuguesas (E-33/11) and MINECO (AIB2010PT-00367, Spain). O.G-S. thanks MINECO for a Formación de Personal Investigador (FPI) predoctoral grant. | - |
dc.publisher | Springer Nature | - |
dc.rights | closedAccess | - |
dc.subject | Selenocysteine | - |
dc.subject | Enzyme kinetics | - |
dc.subject | Isotope exchange | - |
dc.subject | Molecular dynamics | - |
dc.subject | Hydrogenase | - |
dc.title | Influence of the protein structure surrounding the active site on the catalytic activity of [NiFeSe] hydrogenases | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1007/s00775-013-0986-4 | - |
dc.relation.publisherversion | https://doi.org/10.1007/s00775-013-0986-4 | - |
dc.identifier.e-issn | 1432-1327 | - |
dc.date.updated | 2019-05-31T12:02:01Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministério da Educação e Ciência (Portugal) | - |
dc.contributor.funder | Conselho de Reitores das Universidades Portuguesas | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Fundação para a Ciência e a Tecnologia (Portugal) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003381 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001871 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
Aparece en las colecciones: | (ICP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
21
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
20
checked on 22-feb-2024
Page view(s)
170
checked on 17-abr-2024
Download(s)
30
checked on 17-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.