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dc.contributor.authorAntoraz, Sergioes_ES
dc.contributor.authorRico, Sergioes_ES
dc.contributor.authorRodríguez, Héctores_ES
dc.contributor.authorSevillano, Lauraes_ES
dc.contributor.authorAlzate, Juan Fernandoes_ES
dc.contributor.authorSantamaría, Ramón I.es_ES
dc.contributor.authorDíaz, Margaritaes_ES
dc.date.accessioned2019-05-30T12:44:50Z-
dc.date.available2019-05-30T12:44:50Z-
dc.date.issued2017-12-12-
dc.identifier.citationFrontiers in Microbiology 8: 2444 (2017)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/182922-
dc.description.abstractStreptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here, we show how the deletion of the orphan RR Aor1 (SCO2281) provoked a drastic decrease in the production of the three main antibiotics produced by S. coelicolor and a delay in morphological differentiation. With the aim to better understand the transcriptional events underpinning these phenotypes, and the global role of Aor1 in Streptomyces, a transcriptional fingerprint of the Δaor1 mutant was compared to a wild-type strain. RNA-Seq analysis revealed that the deletion of this orphan regulator affects a strikingly high number of genes, such as the genes involved in secondary metabolism, which matches the antibiotic production profiles observed. Of particular note, the sigma factor SigB and all of the genes comprising its regulon were up regulated in the mutant. Our results show that this event links osmotic stress to secondary metabolite production in S. coelicolor and indicates that the RR encoded by aor1 could be a key regulator in both of these processes.es_ES
dc.description.sponsorshipOur laboratory is funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) [PCIN-2014-067 and BIO2015-66958-R]. SA has been supported by a Junta de Castilla y León fellowship (grant CSI099A12-1).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relationMINECO/ICTI2013-2016/PCIN-2014-067es_ES
dc.relationMINECO/ICTI2013-2016/BIO2015-66958-Res_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectStreptomyceses_ES
dc.subjectTwo-component systemses_ES
dc.subjectAntibiotic productiones_ES
dc.subjectOrphan response regulatores_ES
dc.subjectCrosstalkes_ES
dc.subjectStress responsees_ES
dc.titleThe orphan response regulator Aor1 is a new relevant piece in the complex puzzle of Streptomyces coelicolor antibiotic regulatory networkes_ES
dc.typeartículoes_ES
dc.identifier.doi10.3389/fmicb.2017.02444-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2017.02444es_ES
dc.identifier.e-issn1664-302X-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderJunta de Castilla y Leónes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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