English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/182922
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The orphan response regulator Aor1 is a new relevant piece in the complex puzzle of Streptomyces coelicolor antibiotic regulatory network

AuthorsAntoraz, Sergio; Rico, Sergio ; Rodríguez, Héctor ; Sevillano, Laura ; Alzate, Juan Fernando; Santamaría, Ramón I. ; Díaz, Margarita
KeywordsStreptomyces
Two-component systems
Antibiotic production
Orphan response regulator
Crosstalk
Stress response
Issue Date12-Dec-2017
PublisherFrontiers Media
CitationFrontiers in Microbiology 8: 2444 (2017)
AbstractStreptomyces coelicolor, the best-known biological antibiotic producer, encodes 29 predicted orphan response regulators (RR) with a putative role in the response to environmental stimuli. However, their implication in relation to secondary metabolite production is mostly unexplored. Here, we show how the deletion of the orphan RR Aor1 (SCO2281) provoked a drastic decrease in the production of the three main antibiotics produced by S. coelicolor and a delay in morphological differentiation. With the aim to better understand the transcriptional events underpinning these phenotypes, and the global role of Aor1 in Streptomyces, a transcriptional fingerprint of the Δaor1 mutant was compared to a wild-type strain. RNA-Seq analysis revealed that the deletion of this orphan regulator affects a strikingly high number of genes, such as the genes involved in secondary metabolism, which matches the antibiotic production profiles observed. Of particular note, the sigma factor SigB and all of the genes comprising its regulon were up regulated in the mutant. Our results show that this event links osmotic stress to secondary metabolite production in S. coelicolor and indicates that the RR encoded by aor1 could be a key regulator in both of these processes.
Publisher version (URL)https://doi.org/10.3389/fmicb.2017.02444
URIhttp://hdl.handle.net/10261/182922
DOI10.3389/fmicb.2017.02444
E-ISSN1664-302X
Appears in Collections:(IBFG) Artículos
Files in This Item:
File Description SizeFormat 
The Orphan Response Regulator_Antoraz.pdf2,17 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.